LOCUS       sequence-10635-e       10651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Schaffer U, Schlosser A, Muller KM, Schafer A, Katava N, Baumeister 
            R, Schulze E
  TITLE     SnAvi--a new tandem tag for high-affinity protein-complex 
            purification.
  JOURNAL   Nucleic Acids Res. 2010 Apr;38(6):e91. doi: 10.1093/nar/gkp1178. 
            Epub 2010 Jan 4.
  PUBMED    20047968
REFERENCE   2  (bases 1 to 10651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-04"; volume: "38(6):e91. doi"; pages: " 
            10.1093/nar/gkp1178. Epub 2010 Jan 4"
COMMENT     Sequence Label: pBY2946
FEATURES             Location/Qualifiers
     source          1..10651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3748..3759
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5840..5860
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5867..5887
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(5906..5908,5909..5911,5912..6622)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5906..5908,5909..5911,5912..6622)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5951..5972)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6212..6231)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6559..6580
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6680..6724
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     polyA_signal    6849..6970
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6886..6905)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6940..6959
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6977..7432)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7114..7135)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7326..7345
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7459..7563
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7565..7922
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7565..7756
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7588..7608)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7773..7908
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7957..8751
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7957..8751
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8011..8030)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8621..8640
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8939..8958)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8983..9030
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9359..9947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9848..9867
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        10122..10425
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10426..10629
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10580..10600
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctagagaat tttaacaata cttcaaattt gttaagccga acataacaaa aaatgaataa
       61 aaaacggaaa gtgattgacc cgtatttgaa gaagaagaca aatgagtggg ggcgacaatg
      121 ggggaaaaag agacgatgaa agtgaagtgg aattaaatgt cggaaaattt tgtggaaaaa
      181 gaataatgaa atatacatat aatatcagta tcttcagctt ttcctcctgc ttcacaatct
      241 cggcggcaga tttatgtaaa tgatatctcc attcaaatgg tattcaaaga agaagaagaa
      301 gaaggcaatt gctgataatg agataaaagg aggcttctcc tactgataac aattggtcgg
      361 gaattcatga ttagtggaag aaaagggtgg tgttttggat gaagaagatg ttgggaatgc
      421 caatgatcgg atttttgatg caagcagtta tcttttgcaa catgattagt catttaaatc
      481 agggttggcg acaaacaatt tttttccggc aaatcggcaa atccgttagt tgccggaatt
      541 ggtaacttcc ggcaaatcga caaatggtcg gaattggaat gttcggcaaa tcggcaaatc
      601 ggcaatttgt cggaattgaa atttccgaca aagcggcaaa tcggcagttt ttcggaattg
      661 acatttttgg caaatcggca aatcggttag atgccggaac tgaaaacttc cggcaaagcg
      721 gcaaatcgct gactttccaa tttgattttg ttgagaagtt ttatctaaag ttaaatttct
      781 agctaatcgg caagtccgtt aattgccgga attggaaatt tccggcaaat cggcaaagcg
      841 gcacattgtc ggaattgaaa tttctagcta atcggcaaat cggttagttg ccggaattgg
      901 aaacttccgg caaagcgaca aatcacagat tttccaaatt taatttttat gataattttt
      961 tgtaaaaggg ccgaattatt tttgaataat tcaagttttt tttaatttta tggttttttc
     1021 caacattcaa ataaaacata tcaattgaga acattatatt tgaaaaatca tttattggga
     1081 tttgtattga aaagctgaaa aatgagagtt ttcggaatgc attttgtgag aaatatagtg
     1141 aaaaattagg aaattttaaa tttagcggta ggaaaatcac atgatttcta taaattgcga
     1201 gaaaaattat tagttttatt ccatcggcgg gaaattatgc atcatacgag tagtcggcct
     1261 tattgtgcat tacgagctta ttctccacaa aatagaagct atcggagcgg gtctcgtttg
     1321 gattattgat catgtcgtcc actgaaaata tacagttttt tttttaattt tacaacaaaa
     1381 aaacatattt tttttttgca aatttcattt tactttccaa ttagtcagtt tttttccgcg
     1441 ataattttta atttcatggg ctagttttgg caagaattct tctcttcgat gcaccatgag
     1501 cacttaaaaa aaatttgcga acttttttat cggtttaatg tagtttttaa caaaatttct
     1561 caaatgttca atacatttcc agaaattttg aatttcccgt caaataattt tctcagaaaa
     1621 tttgaattct cgcggctaat ttttttctca gaaaatttga attacccgcc aaaaacttct
     1681 cagagaaaac tcgaatttcc tccaagtttt cacattttga actactgtag ggcagctttt
     1741 tgtcaatatt tattttattt ttaaaaacct agaaaattcg ctctattgac aaaatgagct
     1801 caaataattt ttcccgcttc aaactcactg agttgttgtg aaagttgtgg aaattcatag
     1861 atatgttcac agttgtttct cgaatttggc atacagaatc caaattcaaa atcaaagcat
     1921 ttcaataaac gatccttgaa aaagtgccgt tcaatcattc gaaaatgtgt gatcggcaca
     1981 tcgccgacct tgaactcgac cgtcgcgccg accgtcttta atttcagaaa attcggcgca
     2041 aatcgatatc ggacatatct tgccgattcg gcttgcgcct gcagattctc ttccgtctca
     2101 ttttccggtt ttgcaatttc gaagagcacg tgttccgtgt cgaggtcacg gatttggaat
     2161 ttggtgaact caatgttata gacgtttgcc gatggggagc ataggaatcc tgaaaatttg
     2221 atatgtttgt ccacgtggag tacacggcgc gtttatcaat agagcaaata ttcacttacc
     2281 ttgagtgatt cctggaagag ctagcacatc atttggtgta atttgatttt tcgcgagaag
     2341 ctccgcctcg gtggttatag cctgttcggt taccggtggg ggtcggggca tctgaaaaaa
     2401 aaaagagttt tctgtattct gccaaggaaa cagcaaatta tgttcggcaa aaatagacaa
     2461 aaagtaattg agaaaattga ccggtgctgg catgcaattt ttgtacatag atgtgaattt
     2521 agcctatttc aatttattat ttttgtgtga attttttttt taatattcaa acagtgcacg
     2581 cttttttggg aaccaggtgt tggcggcagt tgccgttcgg caaattgcag gtttgccgat
     2641 ttgacgaaac tttgtatttc cggcaatttt ccgttttccg gaaattttta gagaaatatt
     2701 ttataagacg gaaacactta aaactgtgcc tttttgaaat tttttttcgg caaatcaaca
     2761 atttgccaga aactttaaat tccggcaatt tgccaatttg ccgatttgtc ggaaaaaatc
     2821 atttgccgcc aaccccacgt ggtgccaggc tgtcccatca cggtttgata tacaaaaaat
     2881 gcgggaattc tttgtccaaa aagatgtgac gtcagcacgt tcataaaagg ggtgtgcggc
     2941 aaatctcaaa atttgcccag ctcggcaaac ggcaaatttg ccgaacaccc ctggttcata
     3001 accatgtgaa atcagttgag aactctgcgt ctcttctccc gcattttttg tagatcaacg
     3061 tagatcaaac cgaaatggaa cactctgaca ccacgtgcca cccctgattg aaccaccgaa
     3121 aggcacatat acaaatgttc aaaaatgagt ggcaaaaact agcgaaattt gactgcacaa
     3181 aaaaataatt tttaaaattt tcttttaaaa tctactgtgt tattcggtca ttttagcgct
     3241 ataaaagtag attctaatag taagtgcaaa attgcaaatt aaatttattt ggagcaactc
     3301 taagtctctc taaaatccgc gttttttgag aaaagttcaa acaatttcaa cgtagaagaa
     3361 aagaaagcaa gcatatcgat atatgaaatt ggatatcagg agttagtata ctgcgtgcat
     3421 aaacacgctc tctctctcat ttctctgcgt cttcctcacc tgagacggga aggttgccga
     3481 gccgggtgcg atcgattgtt gttgttgctc tgccttcata tatgctgttg tagctgaaaa
     3541 ttttggggat tatgggaatt ttggagagtg ctctggagat tagaggtggt gtggctgcgg
     3601 aaaaaattcg gccaccagta ttttctaggc cgtgggggat agggttattc tttaagcaaa
     3661 tttcaggttt ccaagttttt attgtaaatt ttgagaatta aaataaaaat tataaaagtt
     3721 ctggaaaata tttccgtatg aatttcctgg gaacttcaaa tttttttgta caaagtgcaa
     3781 aaatatattt aatttaaatt ggtttaaaaa attcggccac cagttttttc tatgccgcga
     3841 ggtatagtgt tagtgtgttt ttgacaactt tcaatttttt gagaaaattt taccaaaaat
     3901 attccaagtt ttcatcataa atttcctggg aacttaataa tattttttga gcacaatttg
     3961 gtagaaaata ttttttcaaa ttttcaaatt ttgtaaaatt ggtgaaatat tttgaattca
     4021 ctttcaattt tatttattca aggaatattt tgaaaaaaaa tgtgttttta attcacaaat
     4081 aattactaaa aataactaaa aaataacact gttttatggg atttacagtt tttgtttaaa
     4141 atcctgaaat atttttttta cttaaacatt aataattttt ttatgagtaa agaaatatgc
     4201 aattcaaaaa tatttttaaa atatttgaac gcatccaatt aattgattaa atttatgatg
     4261 tttgcgtttt tttttgagtt tttgaaaaga tatgttccca aaatttttgg aaatgtttgt
     4321 aaaaattttt agatttttta ctccggaaac cctttttatg gaaaactttc aaacccctct
     4381 aatgtaccaa aagagcaaaa aatcaatttt tgcactgaat aaccttttaa ctaactaaaa
     4441 tgaaaaagaa attccaacaa aaaataaatg agttccgtgt gctcttgctc gcagagcaca
     4501 tccaaaaatt aatgggggtc gtccgtaatg attgacagag atggaaaatg gcgaaaaagg
     4561 gctgaagaag atgaaaaaag aagacgtcta ggaaaagaga agcgagagag tgtggggaac
     4621 gctgagggcg cagagagagg aacggacaaa atgacagatg atgagcaaag agaaagagag
     4681 agagggagag agaaagagag ggagagaggg atgaatttgt gatgaaatag acagtgagag
     4741 aaaatgagag gaaaagatgg taattcaata gaatcttaga gcaatttgaa gtttgagtca
     4801 ttacttttaa agtgggcatc tttaaaggta gaatcattta ggattttatc taaataaatc
     4861 tataataaac caatatgact gaatgtcatt ttggtacact gccaaaattc aaaaaaaaat
     4921 tggagaattt gagaaatttt gagctttgct accagtttga acctctggaa caggggtggg
     4981 cggcaattgc cttccggcaa atttcgccaa attgccggtt tgccgatttg tcggaaattt
     5041 taattttcgg caaaaatgcg gatttgctct tttacggata tcaatttgcc ggaagttttt
     5101 agagggattt agtataattc ggaaacactt gaaactgtgc ctttttgaaa ttgtttcccc
     5161 attttcttta gacattttca tagaattcgc ttacttttca aaatagatgt aggaacattc
     5221 ttaggatgcc tacagttttg ccgattaaaa ttgaaattct gaaatttcca aaaaaaaaaa
     5281 ttttaaaacc acaatttgcc attttcgtgt caaaaacttt cggcaattac cgtttttccg
     5341 gcaaattcag caaattggca atttgccggt tccccaattt gccggaaatt ttcagttctg
     5401 gcaatttgcc aatggttttt gaaacatttt acagtaatat ataaggattt catgggtaac
     5461 tataggacta taggactata ggagtggttt aaaaaactga attttgagag acttgacaca
     5521 ttttccaaaa catttttttt aatgttacgt ttgacttaaa gcaatgataa gatatgttaa
     5581 gattttgaag aatttagata tgaggttata gaatactttg cattagtgat gtatttattt
     5641 gaaagaacat tgatggggag aatttttcaa acagaaaaaa aactataaaa ttctacttct
     5701 tttactgaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggttac
     5761 taccatggct ccacgaccat ccaacaaacg tctccagcag tccggtatgc cacgaccctc
     5821 caacaagcgt cttcaacagg agaaccttta ctttcaaggt caattggaaa atctctattt
     5881 ccagggacca ccagcgccac cacagatggt gagcaagggc gaggagctgt tcaccggggt
     5941 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     6001 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     6061 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     6121 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     6181 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     6241 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     6301 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     6361 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     6421 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     6481 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     6541 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     6601 cggcatggac gagctgtaca aggattatga tattccaact actgcaagcg agaatttggc
     6661 agcacagggt gagggtggtg gcctgaacga catcttcgag gcccagaaga tcgagtggca
     6721 cgagtaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     6781 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     6841 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6901 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6961 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     7021 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     7081 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     7141 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     7201 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     7261 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     7321 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     7381 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     7441 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7501 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7561 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     7621 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     7681 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7741 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     7801 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7861 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     7921 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     7981 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     8041 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     8101 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     8161 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     8221 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     8281 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     8341 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     8401 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     8461 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     8521 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     8581 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     8641 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     8701 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     8761 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     8821 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     8881 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     8941 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     9001 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     9061 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     9121 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     9181 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt
     9241 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9301 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9361 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9421 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9481 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     9541 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9601 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9661 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9721 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9781 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9841 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9901 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9961 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    10021 tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca
    10081 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
    10141 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
    10201 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
    10261 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
    10321 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
    10381 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
    10441 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
    10501 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
    10561 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
    10621 agcagagctg gtttagtgaa ccgtcagatc c
//
