LOCUS       sequence-106350-        9231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bait plasmid for an adapted two-hybrid system for C. albicans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Stynen B, Van Dijck P, Tournu H
  TITLE     A CUG codon adapted two-hybrid system for the pathogenic fungus 
            Candida albicans.
  JOURNAL   Nucleic Acids Res. 2010 Oct;38(19):e184. doi: 10.1093/nar/gkq725. 
            Epub 2010 Aug 17.
  PUBMED    20719741
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-10"; volume: "38(19):e184. doi"; pages: " 
            10.1093/nar/gkq725. Epub 2010 Aug 17"
COMMENT     Sequence Label: pC2HB
FEATURES             Location/Qualifiers
     source          1..9231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2595..2614)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2596..2614)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2621..2638)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2621..2637)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2630..2652)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             3864..4166
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4045..4064
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             4515..5309
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4515..5309
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4569..4588)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5179..5198
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      5509..5518
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7021..7041
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7687..7713
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      8462..9050
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8951..8970
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9204..9221
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttggat ccaatcatca ctggtggggg
      361 tcaagaaaga ggtttgagat caggtacttt ggcaccacca ttagtagctg ggtttggtga
      421 agctgcaaga ttaatgaaac aagaatctgc atttgacaag aaacatattg agaggttatc
      481 tactaaattg aagaacggtt tgttatccat tccatctact cagtttaacg gttgtaataa
      541 cccaacatat caatacccag gttgtgtgaa tgtttccttt gcatacattg aaggggaatc
      601 tttgttaatg gcattgaaag atattgcatt gagttctggt tctgcatgta cctctgcatc
      661 tttggaacca tcgtatgttt tacatgcttt gggtgccgat gatgccttgg ctcattcttc
      721 cattagattc ggtattggta gattcaccac tgaagcagaa gttgactatg ttattcaagc
      781 aattaatgaa agagttgatt tcttgagaaa gatgtctcca ttatgggaaa tggttcaaga
      841 aggtattgac ttgaactcta tcgaatggag tggacattaa ggttttttgt ttttttgtaa
      901 tttgtgttgt tatgtgtaat tatcgtgtat agagtaattt cggaaattgt actttcgttc
      961 atgtatctga accagatgaa taatacgttc gtattgttga agaatgacta actcctgtaa
     1021 cggcgtgcgt gggttccgca gccgggttca attttttttt tcttgttcac tataaaaata
     1081 tataataaac gaaaaaaatc cgagggactc aggctatgaa atttttcact caagaatttt
     1141 ttttccttca tccttaattc ctttattcac tacccaaacc cacaatgtca gttaaaacta
     1201 aaactattac aattttacca ggtgatcacg tcggtaccga aattgtcaat gaagctatca
     1261 aagttttaga agcaattgaa gctgccactc cttaccaaaa aatccatttc gatttcaaac
     1321 atcatttaat cggtggtgct gccattgatg ccactggtgt cccattgcca gatgacgcac
     1381 ttgaaagtgc caaaaaatct gatgccgtct tattgggtgc tgttggtgga ccaaaatggg
     1441 ggactggcgc cgttcgtcca gaacaaggtt tattaaagat tcgtaaagaa ttgaaccttt
     1501 atgctaacat cagaccatgt aactttgcca gtgactcctt attggaatta tccccattga
     1561 gaccagaagt cgttaaaggt acaaacttga tcattgttcg tgaattagtt ggtggtattt
     1621 atttcggtga tcgtgaagaa caagaagaaa gtgaagataa acaaaccgca tgggatactg
     1681 aaaaatacac cgtcgatgaa gtcaccagaa tcacccgtat ggctgccttc atggctttac
     1741 aacataaccc accattacca atctggtcat tggataaggc taatgtttta gcttcttcta
     1801 gattatggag aagaactgtc gataaagtga tttctgaaga gttcccaact ttgtctgttc
     1861 aacatcaatt gattgactcc gccgccatga ttttaattca aaacccaacc aaattgaatg
     1921 gtataatcat cacttctaac atgtttggtg atatcatttc cgatgaagct tcagttattc
     1981 caggttcctt gggtttatta ccatctgcat ctttggcttc attgccagat accaacaccg
     2041 cttttggtct ttatgaacca tgtcacggtt ctgctcctga tttaccagcc aataaagtta
     2101 acccaattgc tactatttta tctgctgctt ctatgttgag attatctttg gattgtgtga
     2161 aagaagctga agctttagaa gaagccgtga aacaagtctt ggataaaggt atcagaactg
     2221 cagatttaag aggtaccagt tcagctactg aagttggtga cgcgattgtc gaagctgtta
     2281 ctaaaatctt aaaagaaaaa gcttaagtaa agtatacaca aaatttaacg aatattatta
     2341 atacaaaatg aacaccacct aaatactttt ttttaaaaat gtaccgactc ttctaaaaat
     2401 tgcgataatt caatatcgga aatttctctc accggtttac ttggatcttc ggaaataaat
     2461 ctatttgaag taatggtagc ctcacttgat tctgattggc tctttctaga catgggtagg
     2521 taagggcgaa ttctgcagat atccatcaca ctggcggccg gccgctcgag catgcatcta
     2581 gagggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
     2641 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     2701 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg accagatcaa aatgctagaa
     2761 caaattacgt ctgcatcaac agtcatcttc cttatttgca taaatgctaa aatggcaaac
     2821 ctgtaaaccg tttgaaaagc aaattgagat agttagctcg gttttttttt cacgtggcca
     2881 taatccaaca aaaaaaagga atttaagggg ggagagaagg aggcttgaat ttgtaccaca
     2941 gagcgagcaa gaaattaata atatacttgg cttagctgaa atgagaatga aaaagcaaat
     3001 ttcaagtgaa acaccactac tagccaagta aattggccaa tttgctctcg tgctttaatt
     3061 tcctaacaag cttttgagac cgaaaaaaat caaccaatga tgatggaaaa atacttccgg
     3121 gagtcaattt atccataatt tcacgtcgtt ttttttttgc acatggcaaa tatattggaa
     3181 tcctgagatt ttcttggagt cgtgtacaat aacaccaggc gggaacatgc cgttatgatc
     3241 cagcatagcg gccgctatgt gttcaaatat tgaaaccgtg aaaccagttt gatacagctg
     3301 atattgtgtg ctaaaaaaag atgagcaccg aaagagtctt tagtttattg ccaagtaaag
     3361 ggtcaaagaa tacctattct ttatgaacac agacttgctc taatggtgaa ttagtgtgga
     3421 gtaatagaga aggaggcgaa acaaaaataa ttggttaaac actcgaatgt cacacaatcc
     3481 taaataggtt aaattcagtt accgccgtga tgcgggaaca aagccgagcg gtaatatttt
     3541 tggattctat gtttttttgg tgtatattgc acgacttggt gccaacaact ggtgttccaa
     3601 cgcacaaaac taggttcaac tggatactgt tttctagaaa ttatttgcgg caattcatga
     3661 ttacggtcct aaatttgaaa taaaagtttc caattatgtg tcttgacaat ttccattggg
     3721 ttcaaaaaga tctagaatgt atgtaattac gcaacaattt gcatgcaaag aaataaaaac
     3781 aaacctaaaa tccaactgct gttgttctgg tgatgatgta gtaaaataag agcacggcca
     3841 agaacaaggt gatttacgta cggcagttta aggtttacac ctataaaaga gagagccgtt
     3901 atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga cggatggtga
     3961 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     4021 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     4081 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     4141 ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa aggatcttca
     4201 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
     4261 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
     4321 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
     4381 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     4441 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat
     4501 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4561 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4621 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4681 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4741 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4801 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4861 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4921 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4981 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5041 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5101 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5161 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5221 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5281 tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt
     5341 attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga
     5401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5461 atgagacaat aaccctgata aatgcttcaa taatagcacg tgaggagggc caccatggcc
     5521 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcttccaatt agaggttgtg
     5581 gatgcaaaat tgaaaaaaaa atgactattg gtagcgtgtt ctatttttct tatgcgctga
     5641 aaaactacga acaattgtct attccaagcc tgtggctttt tttggtgatt cacaatacat
     5701 aaattggcaa atacctcttc tatagacttt aacctttgat gaaatggtat ggataaacca
     5761 cttttgtggt cagggtatat atatttttat tccaatctgg cctttataaa ttcaaatttc
     5821 caaggggact ctgctaaacc ttctgatata actataactc taataatggc tgtaaaaagt
     5881 gtgaaaaaaa gatgatattc acaaaaaaga taaaggggat ttcatattgg gtatctagat
     5941 ttaaacatcg tatttcaaca atgaaactag aatcaatacg aaatccaggg cacaactgtg
     6001 ttcattccct tgggaattat actgagtaat gggaagaatg tgcaaaaaat acccgtgtct
     6061 tatataattt ccatttgtgt agggcttaaa agtggacaac ttcaggttac gaaaccacga
     6121 aaatgtctaa acgtttgatc aaattcgtaa ctcatggaaa atcacagcaa caaattaagg
     6181 atggatatat ccggtactta caaataaaaa cactaaagaa gtataaattt tgaactgaaa
     6241 ttctatagaa aatctttttt tttgggtaaa attatgcgat tgtggctcat agtaacgtaa
     6301 tcatacagtt caacaaacat cattttgtca aaggatttct aatcctattc tttttctact
     6361 tggcttttga gtatatatat atatatatat acatcctttg ttcatcatca tgttcgattg
     6421 aatttttttc ttggttgtta ttagattgtc tacttgagtt tttcttggtg caaattgtgg
     6481 tgtttttcta acaaattttt caacatttaa agtatttctg gaacttttct acaaggaacg
     6541 acatagatcc attaatgcgc ccctctaaaa caatacccat aatgtcttct taagtatgca
     6601 tctgcttata aatgtttctg gatttacttt agttagaatg tcgacacgac aatgaactat
     6661 tgctgtagat cacgtgcgat cactgaatta tgagtttctt tttatttttc tttttgggct
     6721 tttttttttg caattttttt tttccacctt tgccaattgc gtagtcacat ataatgtata
     6781 caatttttgc aactctgaca ctataattga tccaataata aagtatataa tagatcatca
     6841 agtatacgta atctccccaa aaaaaaaatt gcataaaata aaatctataa acctttttta
     6901 gatttttagt tgttcctttt ttctttctat tcttcaactt ccaagtgttg tcactttctt
     6961 cctgttaact atatttaaaa gtaaataccc tccccagaaa actccggaat ggctgctcct
     7021 ccaaaaaaga agagaaaggt agctagagaa ttaacaaaac gacaaagcga aatatataac
     7081 tatattaaac aagttgttca aatgaaaggt tatccgccta gtgttcgcga aattggtgag
     7141 gcagttggct tagcatccag ttcaactgtt catggtcacc tttcacgtct tgaagaaaaa
     7201 ggctatataa gaagagatcc aacgaaacca cgtgctatag aaattgtaag tgatcaaaca
     7261 aatgataata ttaatatgga agaaacgatt catgtgccag ttattggtaa agtcacagca
     7321 ggtgttccta ttaccgcagt agaaaatatt gaagaatatt ttccattacc tgaacactta
     7381 acatcgacac acaatagcga catattcata ttaaacgtcg taggcgacag tatgattgag
     7441 gctggtatat tagacggaga caaagtaatt gttcgcagtc aaaccatagc agaaaatgga
     7501 gacattattg ttgctatgac tgaggaagat gaagcaactg tcaaacgctt ctataaagaa
     7561 aaaaatcgtt atcgattaca acctgaaaat agtacaatgg agccaattta cctcgacaat
     7621 gttgctgtaa tcgggaaagt aattggtttg taccgcgaaa tgagatctgg tgctggtggt
     7681 gttggttatc cgtatgatgt gcctgactac gcaggtgctg gtggtgttgg tgctagcagg
     7741 cctttcgaaa ggcgcgccac gcgtagtact taaccgcgcc gtcgacgagt gaaattctgg
     7801 aaatctggaa atctggtttt gtattcttgt tattcttctt tttgttatta catatataac
     7861 ttgttacttt tttaaaaaaa tctttgttta ttttataaat atataaaact aaatttaaga
     7921 aaaagagaaa aatgttttat ttgagagatt gaaattttac ttgaatttag cttagctttt
     7981 ataaagtatt attatgtaaa aaaacaaaac aaatatacat taaaaagtta agactataaa
     8041 atagccaccc aaggcatttc tatatcttgt tgttgttgtt ttcatcttct gtatcagagg
     8101 aacttatttt attattttcg tcacgggtat tttctcttgt ttgatgattc atcccattca
     8161 ttccatcata aaattctaga aggaccacct ttgattgtaa atagtaataa ttaccaccct
     8221 tatttaatta tttatttaac ttatttattt atttattata catatataca aatctaataa
     8281 agtgaaaatc tcccccttca cacgtggccg aggagcagga ctgacacgtg ctaaaacttc
     8341 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8401 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8461 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8521 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8581 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     8641 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8701 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8761 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8821 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8881 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8941 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9001 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9061 acgcggcctt tttacggttc ctgggctttt gctggccttt tgctcacatg ttctttcctg
     9121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa g
//
