LOCUS       sequence-106353-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA against human pro-caspase 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Doitsh G, Galloway NL, Geng X, Yang Z, Monroe KM, Zepeda O, Hunt PW,
            Hatano H, Sowinski S, Munoz-Arias I, Greene WC
  TITLE     Cell death by pyroptosis drives CD4 T-cell depletion in HIV-1 
            infection.
  JOURNAL   Nature. 2014 Jan 23;505(7484):509-14. doi: 10.1038/nature12940.
  PUBMED    24356306
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2014-01-23- 23"; volume: "505"; issue: "7484"; 
            pages: "509-14"
COMMENT     Sequence Label: shRNA Caspase 1 (pSicoR mCherry)
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(285..318)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        518..729
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
       61 cagtttggtt agtaccgggc ccgctctaga gatccgacgc gccatctcta ggcccgcgcc
      121 ggccccctcg cacagacttg tgggagaagc tcggctactc ccctgccccg gttaatttgc
      181 atataatatt tcctagtaac tatagaggct taatgtgcga taaaagacag ataatctgtt
      241 ctttttaata ctagctacat tttacatgat aggcttggat ttctataact tcgtatagca
      301 tacattatac gaagttatac atgtcacaaa aggaaactca ccctaactgt aaagtaattg
      361 tgtgttttga gactataaat atcccttgga gaaaagcctt gtttacacgt cttgctctca
      421 ttattcaaga gataatgaga gcaagacgtg tttttttctc gagtactagg atccattagg
      481 cggccgcgga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
      541 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
      601 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
      661 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
      721 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
      781 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
      841 tgaactgcgt ccgccgtcta ggtaagttta aagctcangt cgagaccggg cctttgtccg
      901 gcgctccctt gnnncctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
      961 gctcaactct acgtctttgt ttcgttttct g
//
