LOCUS       sequence-106360-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp). attP upstream of attB
            in this plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_13_switchBP
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(118..152)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     207..224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(248..305)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(333..360)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(376..447)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aggcctgcag caaacgacga aaactacgct taatattcgg agttgtattt atgttactaa
       61 aacaactggt caagttctac aaatacaacc gttattggtt atcgctctca tcgaactgct
      121 agcattatac ctaggactga gctagctgtc aaattaccgc ctttgagtga gctgataccg
      181 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc tgcataacgc
      241 gaagtaatct tttcggtttt aaagaaaaag ggcagggtgg tgacaccttg cccttttttg
      301 ccggactgca gctgccgcta ctagtatata aacgcagaaa ggcccacccg aaggtgagcc
      361 agtgtgactc tagtagagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
      421 tttcgactga gcctttcgtt ttatttgatg cctggctcta gtattattaa gcaccggtgg
      481 agtgacgacc ttcagcacgt tcgtactgtt caacgatggt gtagtcttcg ttgtgggagg
      541 tgatgtccag tttgatgtcg gttttgtaag cacccggcag ctgaaccggt tttttagcca
      601 tgtaggtggt tttaacttca gcgtcgtagt gaccaccgtc tttcagtttc agacgcattt
      661 tgatttcacc tttcagagca ccgtcttccg ggtacatacg ttcggtggaa gcttcccaac
      721 ccatggtttt
//
