LOCUS       sequence-106362-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as
            well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites 
            of Int7. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pCas_5+7_gfp
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(121..155)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     terminator      273..344
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      360..387
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        456..505
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
ORIGIN
        1 aaggcctgca gcaaacgacg aaaactacgc ttaaaaggaa cggacgaccc ggtcatcaac
       61 tttgcccaac tgacccagac ggacggaacg tttctcgtct gttatcgctc tcatcgaact
      121 gctagcatta tacctaggac tgagctagct gtcaaattac cgcctttgag tgagctgata
      181 ccgtgaggtc tgcctcgtgg gtctctacct aaaaaaaacc ccgccctgtc aggggcgggg
      241 tttttttttg ggtatgggcc ctagccaggc atcaaataaa acgaaaggct cagtcgaaag
      301 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      361 gctcaccttc gggtgggcct ttctgcgttt ataccctaat acgcaagtcg ataactctcc
      421 tgggagcgtt gacaacttgc gcaccctgat ctgagctgtc accggatgtg ctttccggtc
      481 tgatgagtcc gtgaggacga aacagcctct acaaataatt ttgtttaaag taatttcaac
      541 aaaataacta ggattcgaat gaaagtggcc atttatgttc gtgttagcac cgatgaacag
      601 gccaaagaag gttttagcat tccggcacag cgtgaacgtc tgcgtgcatt ttgtgcaagc
      661 cagggttggg aaattgtgca agaatatatt gaagaaggtt ggagcgcaaa agatctggat
      721 cgtccgcaga tgcagcgtct gctgaaagat atcaaaaaag gcaacattga tattgtgctg
      781 gtgtatcgtc tggatcgcct gacccgtagc gttctggatc tgtatctgct gctgca
//
