LOCUS       sequence-106366-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_4
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(130..164)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     219..236
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(260..317)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(345..372)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(388..459)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 actatacaaa aggcctgcag caaacgacga aaactacgct taaaatcatc gcctttacac
       61 aagcagcagt cttattcaaa tttcaggtcg cgttgggcgc tctttggaag ttatcgctct
      121 catcgaactg ctagcattat acctaggact gagctagctg tcaaattacc gcctttgagt
      181 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      241 cctgcataac gcgaagtaat cttttcggtt ttaaagaaaa agggcagggt ggtgacacct
      301 tgcccttttt tgccggactg cagctgccgc tactagtata taaacgcaga aaggcccacc
      361 cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaacaac agataaaacg
      421 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggctc tagtattatt
      481 aagcaccggt ggagtgacga ccttcagcac gttcgtactg ttcaacgatg gtgtagtctt
      541 cgttgtggga ggtgatgtcc agtttgatgt cggttttgta agcacccggc agctgaaccg
      601 gttttttagc catgtaggtg gttttaactt cagcgtcgta gtgaccaccg tctttcagtt
      661 tcagacgcat tttgatttca cctttcagag caccgtcttc cgggtacata cgttcggtgg
      721 aagcttccca acccatggtt ttt
//
