LOCUS       sequence-106368-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_10
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(121..155)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(251..308)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(336..363)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(379..450)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaaggcctgc agcaaacgac gaaaactacg cttaaaagct aaggttaaag cttttacatt
       61 ggtggaaatg ttggtggtct tgctgattat cagcgtgctt gttatcgctc tcatcgaact
      121 gctagcatta tacctaggac tgagctagct gtcaaattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcctgcataa
      241 cgcgaagtaa tcttttcggt tttaaagaaa aagggcaggg tggtgacacc ttgccctttt
      301 ttgccggact gcagctgccg ctactagtat ataaacgcag aaaggcccac ccgaaggtga
      361 gccagtgtga ctctagtaga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
      421 gtctttcgac tgagcctttc gttttatttg atgcctggct ctagtattat taagcaccgg
      481 tggagtgacg accttcagca cgttcgtact gttcaacgat ggtgtagtct tcgttgtggg
      541 aggtgatgtc cagtttgatg tcggttttgt aagcacccgg cagctgaacc ggttttttag
      601 ccatgtaggt ggttttaact tcagcgtcgt agtgaccacc gtctttcagt ttcagacgca
      661 ttttgatttc acctttcaga gcaccgtctt ccgggtacat acgttcggtg gaagcttccc
      721 aacccatggt ttttttctgc ataaccggac cgtcggacgg gaagttggta ccacgcagtt
      781 taactttgta gatgaactca ccgtcttgca gggaggagtc ctgggtaacg gtaacaacac
      841 caccgtcttc gaagttcata acacgttcc
//
