LOCUS       sequence-106375-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp). attP upstream of attB
            in this plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_13_switchBP
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..23
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(54..103)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     terminator      complement(184..270)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(505..522)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(719..738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcatctagt atttctcctc tttctctaga ttaaacaaaa ttatttgtag aggctgtttc
       61 gtcctcacgg actcatcaga ccggaaagca catccggtga cagctactca catggattgc
      121 ttatatttac aggaccaact ggatctggaa aaacaacaac aatgtatgcg tctagggcgg
      181 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
      241 gtctttcgac tgagcctttc gttttatttg atgccacgcg taccatggga ctagggccca
      301 tacccaaaaa aaaaccccgc ccctgacagg gcggggtttt ttttaggtag agacccacga
      361 ggcagacctc agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg
      421 tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat
      481 gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact
      541 gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat
      601 acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct
      661 gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa
      721 agataccagg cgtttccc
//
