LOCUS       sequence-106379-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as
            well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites 
            of Int7. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pCas_5+7_gfp
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..23
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(54..103)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     terminator      complement(216..302)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(537..554)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(751..770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcatctagt atttctcctc tttctctaga ttaaacaaaa ttatttgtag aggctgtttc
       61 gtcctcacgg actcatcaga ccggaaagca catccggtga cagctaagct gaacctgcgt
      121 aaaagttaag gttaggcatg taaacttaac tctcacacag gtttataaca ccacagagga
      181 cacatgccct acctccatga tctctagggc ggcggatttg tcctactcag gagagcgttc
      241 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
      301 tgatgccacg cgtaccatgg gactagggcc catacccaaa aaaaaacccc gcccctgaca
      361 gggcggggtt ttttttaggt agagacccac gaggcagacc tcagcgctag cggagtgtat
      421 actggcttac tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga
      481 aaaaaggctg caccggtgcg tcagcagaat atgtgataca ggatatattc cgcttcctcg
      541 ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg
      601 ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg
      661 caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa
      721 atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
//
