LOCUS       sequence-106380-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lenti vector for expression of inducible split Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: pLSC-5
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             289..336
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             346..402
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc
       61 gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg
      121 tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac
      181 cggaagaggt acaccagcac caaagaggtg ctggatgcca ccctgatcca ccagagcatc
      241 accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgacaa aaggccggcg
      301 gccacgaaaa aggccggcca ggcaaaaaag aaaaagggat ccggcgcaac aaacttctct
      361 ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgctagattt agctagctta
      421 atcctcgaca agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc
      481 gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac
      541 cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc
      601 gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc
      661 tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac
      721 agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc
      781 ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga
      841 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc
      901 cacatgatca agttccgggg ccactt
//
