LOCUS       sequence-106381-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lenti vector for expression of inducible split Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: pLSC-5
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             108..128
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             153..473
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
       61 tcgcaacggg tttgccgcca gaacacagga ccggtgccac catggcccca aagaagaagc
      121 ggaaggtcgg tatccacgga gtcccagcag ccggcgtgca ggtggagacc atcagccccg
      181 gcgacggcag aaccttcccc aagagaggcc agacctgcgt ggtgcactac accggcatgc
      241 tggaggacgg caagaagttc gacagcagca gagacagaaa caagcccttc aagttcatgc
      301 tgggcaagca ggaggtgatc agaggctggg aggagggcgt ggcccagatg agcgtgggcc
      361 agagagccaa gctgaccatc agccccgact acgcctacgg cgccaccggc caccccggca
      421 tcatccctcc tcacgccacc ctggtgttcg acgtggagct gctgaagctg gagggtggcg
      481 gtggctcggg tggcggtggc tcgggtggcg gtggctcgtg cttcgactcc gtggaaatct
      541 ccggcgtgga agatcggttc aacgcctccc tgggcgcata ccacgatctg ctgaaaatta
      601 tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc
      661 tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc
      721 acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca
      781 ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg
      841 atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca
      901 gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc
      961 acgagcacat tgccaatctg gccggcagcc c
//
