LOCUS       sequence-106382-         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lenti vector for expression of inducible split Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: pLSC-5
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..641)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(128..148)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 tcacaaattt tgtaatccag aggttgattg tcgacttaac gcgttcaggc accgggcttg
       61 cgggtcatgc accaggtgcg cggtccttcg ggcacctcga cgtcggcggt gacggtgaag
      121 ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc
      181 ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc
      241 tggtggtcgg gcgagactcc gacggtggcc aggaaccacg cgggctcctt gggccggtgc
      301 ggcgccagga ggccttccat ctgttgctgc gcggccagcc gggaaccgct caactcggcc
      361 atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga cgctctccgg cgtggtccag
      421 accgccaccg cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc
      481 gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg atcgacggtg
      541 tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc cctggggacg
      601 tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg tc
//
