LOCUS       sequence-106386-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: PX851
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..279)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
ORIGIN
        1 gaattcttac tgcttgctga ttcttctgaa cacgtggtag tacaggtccc aggcctgggt
       61 caggtccttc acgttgccgc tcttcatgta ctttctgcac cactcctggg cctccatcag
      121 gtctctgccg taggcctggt tgaagctggt ctccttcagg gtctgggggc ctctctccat
      181 catggcgtgc aggggctcca gcacctcgaa catgcccttc acgtttctct cgccgaagta
      241 cagtctgctg gcctcctcca ggccctcgtg ccacatctcc gagccaccgc cacccgagcc
      301 accgccaccc gagccaccgc cacctctcat tccctcggtc acgtatttca ctttggtcag
      361 ctcgttatac acggtgaagt actcgtacag caggctgtgc ttgggcagca ccttctcgtt
      421 gggcaggttc ttatcgaagt tggtcatccg ctcgatgaag ctctgggcgg aagcgccctt
      481 gtccaccact tcctcgaagt tccagggggt gatggtttcc tcgctctttc tggtcatcca
      541 ggcgaatctg ctgtttcccc tggccagagg gcccacgtag taggggatgc ggaaggtcag
      601 gatcttctcg atcttttccc ggttgtcctt caggaatggg taaaaatctt cctgccgccg
      661 cagaatggcg tgcagctctc ccaggtggat ctggtggggg atgctgccgt tgtcgaaggt
      721 ccgctgcttc cgcagcaggt cctctctgtt cagcttcacg agcagttcct cggtgccgtc
      781 catcttttcc aggatgggct tgatgaactt gtagaactct tcctggctgg ctccgccgtc
      841 aatgtagccg gcgtagccgt tcttgctctg gtcgaagaaa
//
