LOCUS       sequence-106392-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: PX852
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             65..85
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             110..430
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggaccg gtgccaccat
       61 ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg gcgtgcaggt
      121 ggagaccatc agccccggcg acggcagaac cttccccaag agaggccaga cctgcgtggt
      181 gcactacacc ggcatgctgg aggacggcaa gaagttcgac agcagcagag acagaaacaa
      241 gcccttcaag ttcatgctgg gcaagcagga ggtgatcaga ggctgggagg agggcgtggc
      301 ccagatgagc gtgggccaga gagccaagct gaccatcagc cccgactacg cctacggcgc
      361 caccggccac cccggcatca tccctcctca cgccaccctg gtgttcgacg tggagctgct
      421 gaagctggag ggtggcggtg gctcgggtgg cggtggctcg ggtggcggtg gctcgaagcc
      481 cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg
      541 gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc
      601 cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct
      661 gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga
      721 agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa
      781 aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac
      841 cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa
      901 gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat
      961 ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggcc
//
