LOCUS       sequence-106394-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a modified version of the pGL4.23 vector from Promega, 
            containing a Gateway cassette upstream of the minimal promoter. This
            vector can be used for for Gateway cloning of candidate enhancer 
            elements. As negative control, use pGL4.23 without the gateway 
            cassette (available from Promega)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pGL4.23-GW
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(103..227)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        301..332
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      359..368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
       61 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
      121 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
      181 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacccgctg
      241 atcagcctcg actgtgctcg aggatatcaa gatctggcct cggcggccaa gcttagacac
      301 tagagggtat ataatggaag ctcgacttcc agcttggcaa tccggtactg ttggtaaagc
      361 caccatggaa gatgccaaaa acattaagaa gggcccagcg ccattctacc cactcgaaga
      421 cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg tgcccggcac
      481 catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt acttcgagat
      541 gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc atcggatcgt
      601 ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc tgttcatcgg
      661 tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga acagcatggg
      721 catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga tcctcaacgt
      781 gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga ccgactacca
      841 gggcttccaa agcatgtaca ccttcgtgac ttcccat
//
