LOCUS       sequence-106396-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a modified version of the pGL4.23 vector from Promega, 
            containing a Gateway cassette upstream of the minimal promoter. This
            vector can be used for for Gateway cloning of candidate enhancer 
            elements. As negative control, use pGL4.23 without the gateway 
            cassette (available from Promega)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pGL4.23-GW
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    44..168
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(193..198,199..216,217..223)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(343..362)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctggccggta cctgagctcc cgtcgacgaa ttctgcagat atcacaagtt tgtacaaaaa
       61 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
      121 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta
      181 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
      241 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
      301 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
      361 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
      421 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
      481 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
      541 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
      601 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
      661 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
      721 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
      781 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
      841 attcaggttc atcatgccgt ttgtgatggc ttccatgt
//
