LOCUS       sequence-106400-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term SpCas9 piece of inducible transcriptional activator 
            (dCas9(N)-FRB-2xNES).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: PX855
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..303)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
ORIGIN
        1 gaattcttag aggattaagc tagctaaatc tagctgcttg ctgattcttc tgaacacgtg
       61 gtagtacagg tcccaggcct gggtcaggtc cttcacgttg ccgctcttca tgtactttct
      121 gcaccactcc tgggcctcca tcaggtctct gccgtaggcc tggttgaagc tggtctcctt
      181 cagggtctgg gggcctctct ccatcatggc gtgcaggggc tccagcacct cgaacatgcc
      241 cttcacgttt ctctcgccga agtacagtct gctggcctcc tccaggccct cgtgccacat
      301 ctccgagcca ccgccacccg agccaccgcc acccgagcca ccgccacctc tcattccctc
      361 ggtcacgtat ttcactttgg tcagctcgtt atacacggtg aagtactcgt acagcaggct
      421 gtgcttgggc agcaccttct cgttgggcag gttcttatcg aagttggtca tccgctcgat
      481 gaagctctgg gcggaagcgc ccttgtccac cacttcctcg aagttccagg gggtgatggt
      541 ttcctcgctc tttctggtca tccaggcgaa tctgctgttt cccctggcca gagggcccac
      601 gtagtagggg atgcggaagg tcaggatctt ctcgatcttt tcccggttgt ccttcaggaa
      661 tgggtaaaaa tcttcctgcc gccgcagaat ggcgtgcagc tctcccaggt ggatctggtg
      721 ggggatgctg ccgttgtcga aggtccgctg cttccgcagc aggtcctctc tgttcagctt
      781 cacgagcagt tcctcggtgc cgtccatctt ttccaggatg ggcttgatga acttgtagaa
      841 ctcttcctgg ctggctccgc cgtcaatgta gccggcgtag ccgttcttgc tctggtcgaa
      901 gaaaa
//
