LOCUS       sequence-106406-        2176 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Mondragon A, Griffith EC, Sun L, Xiong F, Armstrong C, Liu JO
  TITLE     Overexpression and purification of human calcineurin alpha from 
            Escherichia coli and assessment of catalytic functions of residues 
            surrounding the binuclear metal center.
  JOURNAL   Biochemistry. 1997 Apr 22. 36(16):4934-42.
  PUBMED    9125515
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 1997 Apr 22. 36(16):4934-42."
COMMENT     Sequence Label: pET15b CnA CnB
FEATURES             Location/Qualifiers
     source          1..2176
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..32
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             42..59
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccatgggcag cagccatcat catcatcatc acagcagcgg cctggtgccg cgcggcagcc
       61 atatgtccga gcccaaggca attgatccca agttgtcgac gaccgacagg gtggtgaaag
      121 ctgttccatt tcctccaagt caccggctta cagcaaaaga agtgtttgat aatgatggaa
      181 aacctcgtgt ggatatctta aaggcgcatc ttatgaagga gggaaggctg gaagagagtg
      241 ttgcattgag aataataaca gagggtgcat caattcttcg acaggaaaaa aatttgctgg
      301 atattgatgc gccagtcact gtttgtgggg acattcatgg acaattcttt gatttgatga
      361 agctctttga agtcggggga tctcctgcca acactcgcta cctcttctta ggggactatg
      421 ttgacagagg gtacttcagt attgaatgtg tgctgtattt gtgggccttg aaaattctct
      481 accccaaaac actgttttta cttcgtggaa atcatgaatg tagacatcta acagagtatt
      541 tcacatttaa acaagaatgt aaaataaagt attcagaacg cgtatatgat gcctgtatgg
      601 atgcctttga ctgccttccc ctggctgccc tgatgaacca acagttcctg tgtgtgcatg
      661 gtggtttgtc tccagagatt aacactttag atgatatcag aaaattagac cgattcaaag
      721 aaccacctgc atatggacct atgtgtgata tcctgtggtc agaccccctg gaagattttg
      781 gaaatgagaa gactcaggaa catttcactc acaacacagt cagggggtgt tcatacttct
      841 acagttaccc ggctgtatgt gaattcttac agcacaataa cttgttatct atactccgag
      901 cccacgaagc ccaagatgca gggtaccgca tgtacaggaa aagccaaaca acaggcttcc
      961 cttctctaat tacaattttt tcagcaccaa attacttaga tgtatacaat aacaaagctg
     1021 cagtattgaa gtatgagaac aatgttatga atatcaggca attcaactgt tctcctcatc
     1081 catactggct tccaaatttc atggatgttt ttacttggtc ccttccattt gttggggaaa
     1141 aagtgactga gatgctggta aatgtcctca acatctgctc agatgatgaa ctagggtcag
     1201 aagaagatgg atttgatggt gcaacagctg cagcccggaa agaggtgata aggaacaaga
     1261 tccgagcaat aggcaaaatg gccagagtgt tctcagtgct cagagaagag agtgagagtg
     1321 tgctgacgct gaaaggcttg accccaactg gcatgctccc cagcggagta ctttctggag
     1381 ggaagcaaac cctgcaaagc gctactgttg aggctattga ggctgatgaa gctatcaaag
     1441 gattttcacc acaacataag atcactagct tcgaggaagc caagggctta gaccgaatta
     1501 atgagaggat gccgcctcgc agagatgcca tgccctctga cgccaacctt aactccatca
     1561 acaaggctct cacctcagag actaacggca cggacagcaa tggcagtaat agcagcaata
     1621 ttcagtgact cgaggagata tacatatggg aaatgaggca agttatcctt tggaaatgtg
     1681 ctcacacttt gatgcggatg aaattaaaag gctaggaaag agatttaaga agcttgattt
     1741 ggataattct ggttctttga gtgtggaaga gttcatgtct ctgcctgagt tacaacagaa
     1801 tcctttagta cagcgagtaa tagatatatt cgacacagat gggaatggag aagtagactt
     1861 taaagaattc attgagggcg tctctcagtt cagtgtcaaa ggagataagg agcagaaatt
     1921 gaggtttgct ttccgtatct atgacatgga taaagatggc tatatttcca atggggaact
     1981 cttccaggta ttgaagatga tggtggggaa caatctgaaa gatacacagt tacagcaaat
     2041 tgtagacaaa accataataa atgcagataa ggatggagat ggaagaatat cctttgaaga
     2101 attctgtgct gttgtaggtg gcctagatat ccacaaaaag atggtggtag atgtgtgact
     2161 gcagcccggg ggatcc
//
