LOCUS       sequence-10641-e         451 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Hubner A, Barrett T, Flavell RA, Davis RJ
  TITLE     Multisite phosphorylation regulates Bim stability and apoptotic 
            activity.
  JOURNAL   Mol Cell. 2008 May 23. 30(4):415-25.
  PUBMED    18498746
REFERENCE   2  (bases 1 to 451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 May 23. 30(4):415-25."
COMMENT     Sequence Label: pCMV-Tag2B Flag BimS
FEATURES             Location/Qualifiers
     source          1..451
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..30
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      65..74
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             74..97
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gccaagctcg aaattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc
       61 ggccgccacc atggattaca aggatgacga cgataagagc ccgggcggat ccatggccaa
      121 gcaaccttct gatgtaagtt ctgagtgtga cagagaaggt ggacaattgc agcctgctga
      181 gaggcctccc cagctcaggc ctggggcccc tacctcccta cagacagaac cgcaagcttc
      241 cataagacag tctcaagagg aacctgaaga tctgcgcccg gagatacgga ttgcacagga
      301 gctacggcgg atcggagacg agttcaacga aacttacaca aggagggtgt ttgcaaatga
      361 ttaccgcgag gctgaagacc accctcaaat ggttatctta caactgttac gctttatctt
      421 ccgtctggta tggagaaggc attgagaatt c
//
