LOCUS       sequence-106410-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Beronja S, Livshits G, Williams S, Fuchs E
  TITLE     Rapid functional dissection of genetic networks via tissue-specific 
            transduction and RNAi in mouse embryos.
  JOURNAL   Nat Med. 2010 Jul . 16(7):821-7.
  PUBMED    20526348
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2010 Jul . 16(7):821-7."
COMMENT     Sequence Label: LV-GFP
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..431
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      444..453
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(495..516)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatct accatgccag
       61 agccagcgaa gtctgctccc gccccgaaaa agggctccaa gaaggcggtg actaaggcgc
      121 agaagaaagg cggcaagaag cgcaagcgca gccgcaagga gagctattcc atctatgtgt
      181 acaaggttct gaagcaggtc caccctgaca ccggcatttc gtccaaggcc atgggcatca
      241 tgaactcgtt tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc
      301 attacaacaa gcgctcgacc atcacctcca gggagatcca gacggccgtg cgcctgctgc
      361 tgcctgggga gttggccaag cacgccgtgt ccgagggtac taaggccatc accaagtaca
      421 ccagcgctaa ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
      481 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cngccacaag ttcagcgtgt
      541 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      601 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      661 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      721 aaggctacgt ccagga
//
