LOCUS       sequence-106412-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFA6a -mVenus-BirA::HIS5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Jan CH, Williams CC, Weissman JS
  TITLE     Principles of ER cotranslational translocation revealed by 
            proximity-specific ribosome profiling.
  JOURNAL   Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521.
            Epub 2014 Nov 6.
  PUBMED    25378630
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-7"; volume: "346(6210):1257521. doi"; pages: " 
            10.1126/science.1257521. Epub 2014 Nov 6"
COMMENT     Sequence Label: pJW1507
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..761
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             48..761
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 cgtacgctgc aggtcgacgg atcggtgacg gtgctggttt aattaacatg tctaaaggtg
       61 aagaattatt cactggtgtt gtcccaattt tggttgaatt agatggtgat gttaatggtc
      121 acaaattttc tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa
      181 aattgatttg tactactggt aaattgccag ttccatggcc aaccttagtc actactttag
      241 gttatggttt gcaatgtttt gctagatacc cagatcatat gaaacaacat gactttttca
      301 agtctgccat gccagaaggt tatgttcaag aaagaactat ttttttcaaa gatgacggta
      361 actacaagac cagagctgaa gtcaagtttg aaggtgatac cttagttaat agaatcgaat
      421 taaaaggtat tgattttaaa gaagatggta acattttagg tcacaaattg gaatacaact
      481 ataactctca caatgtttac atcactgctg acaaacaaaa gaatggtatc aaagctaact
      541 tcaaaattag acacaacatt gaagatggtg gtgttcaatt agctgaccat tatcaacaaa
      601 atactccaat tggtgatggt ccagtcttgt taccagacaa ccattactta tcctatcaat
      661 ctaagttatc caaagatcca aacgaaaaga gagaccacat ggtcttgtta gaatttgtta
      721 ctgctgctgg tattacccat ggtatggatg aattgtacaa aggtggtagt ggtggtagtg
      781 gtatgaagga taacaccgtg ccactgaaat tgattgccct gttagcgaac ggtgaatt
//
