LOCUS       sequence-106417-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_4
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..23
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(54..103)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     terminator      complement(175..202)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(218..289)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(512..529)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(726..745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcatctagt atttctcctc tttctctaga ttaaacaaaa ttatttgtag aggctgtttc
       61 gtcctcacgg actcatcaga ccggaaagca catccggtga cagctaaacg aattacaaat
      121 tatttgaccg ctaattcaaa tcaagggttg aaaactttgt aatttttgta taaacgcaga
      181 aaggcccacc cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaacaac
      241 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcta
      301 gggcccatac ccaaaaaaaa accccgcccc tgacagggcg gggttttttt taggtagaga
      361 cccacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt tggcactgat
      421 gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc
      481 agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg
      541 ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca
      601 ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg
      661 cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
      721 actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc
      781 ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgc
//
