LOCUS       sequence-106419-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_8
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..23
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(54..103)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     terminator      complement(176..203)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(219..290)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(513..530)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(727..746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcatctagt atttctcctc tttctctaga ttaaacaaaa ttatttgtag aggctgtttc
       61 gtcctcacgg actcatcaga ccggaaagca catccggtga cagctatttt attatggaag
      121 tttgttcact caacattgca agactgtaca tacttccata gtttattaat ataaacgcag
      181 aaaggcccac ccgaaggtga gccagtgtga ctctagtaga gagcgttcac cgacaaacaa
      241 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggct
      301 agggcccata cccaaaaaaa aaccccgccc ctgacagggc ggggtttttt ttaggtagag
      361 acccacgagg cagacctcag cgctagcgga gtgtatactg gcttactatg ttggcactga
      421 tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag
      481 cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc
      541 gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc
      601 aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc
      661 gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag
      721 gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg
      781 cctttcggtt taccggtgtc attccgctgt tatggccgcg tt
//
