LOCUS       sequence-106421-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_10
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..23
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(54..103)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     terminator      complement(215..301)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(536..553)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(750..769)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcatctagt atttctcctc tttctctaga ttaaacaaaa ttatttgtag aggctgtttc
       61 gtcctcacgg actcatcaga ccggaaagca catccggtga cagctaaaag agtgagagtt
      121 ttactatcct tgattgagat gtaggttact aaaattattt atattttcca aacagaggac
      181 acatgcccta cctccatgat ctctagggcg gcggatttgt cctactcagg agagcgttca
      241 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      301 gatgccacgc gtaccatggg actagggccc atacccaaaa aaaaaccccg cccctgacag
      361 ggcggggttt tttttaggta gagacccacg aggcagacct cagcgctagc ggagtgtata
      421 ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa
      481 aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc
      541 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg
      601 gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc
      661 aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa
      721 tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc
      781 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
      841 gcgtt
//
