LOCUS       sequence-106424-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used with pAND_YFP  to generate an AND gate without memory .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pSpec
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..51
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    58..76
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             114..791
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 ttgataacaa aatactccac cgttggcttt tttccctatc agtgatagag attgacatcc
       61 ctatcagtga tagagataat gagcactact agagtcacac aggaaagtac tagatggctt
      121 cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta
      181 acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga
      241 ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc
      301 cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc
      361 tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg
      421 gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta
      481 aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt
      541 gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa
      601 tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg
      661 ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct
      721 cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca
      781 ccggtgctta ataatactag agccaggcat caa
//
