LOCUS       sequence-106434-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal 3X FLAG fused to wt BirA - mCherry - sec61ß.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Jan CH, Williams CC, Weissman JS
  TITLE     Principles of ER cotranslational translocation revealed by 
            proximity-specific ribosome profiling.
  JOURNAL   Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521.
            Epub 2014 Nov 6.
  PUBMED    25378630
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-7"; volume: "346(6210):1257521. doi"; pages: " 
            10.1126/science.1257521. Epub 2014 Nov 6"
COMMENT     Sequence Label: pJW1512
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             61..126
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctaccggt cgccaccatg
       61 gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat
      121 gacaagctta tgaaggataa caccgtgcca ctgaaattga ttgccctgtt agcgaacggt
      181 gaatttcact ctggcgagca gttgggtgaa acgctgggaa tgagccgggc ggctattaat
      241 aaacacattc agacactgcg tgactggggc gttgatgtct ttaccgttcc gggtaaagga
      301 tacagcctgc ctgagcctat ccagttactt aatgctaaac agatattggg tcagctggat
      361 ggcggtagtg tagccgtgct gccagtgatt gactccacga atcagtacct tcttgatcgt
      421 atcggagagc ttaaatcggg cgatgcttgc attgcagaat accagcaggc tggccgtggt
      481 cgccggggtc ggaaatggtt ttcgcctttt ggcgcaaact tatatttgtc gatgttctgg
      541 cgtctggaac aaggcccggc ggcggcgatt ggtttaagtc tggttatcgg tatcgtgatg
      601 gcggaagtat tacgcaagct gggtgcagat aaagttcgtg ttaaatggcc taatgacctc
      661 tatctgcagg atcgcaagct ggcaggcatt ctggtggagc tgactggcaa aactggcgat
      721 gcggcgcaaa tagtcattgg agccgggatc aacatggcaa tgcgccgtgt tgaagagagt
      781 gtcgttaatc aggggtggat cacgctgcag gaagcgggga tcaatctcga tcgtaatacg
      841 ttggcggcca tgctaatacg tgaattacgt gctgcgttgg aactcttcga acaagaa
//
