LOCUS       sequence-106440-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: pENTR-BsaI
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(154..159,160..177,178..184)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(304..323)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc tgagaccgcg
      121 gcccctcgag gggtcgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      181 aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg
      241 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
      301 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
      361 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
      421 attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag
      481 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
      541 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
      601 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
      661 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
      721 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
      781 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc
      841 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt g
//
