LOCUS       sequence-106445-         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  aTc inducible gRNA expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pRPR1(1xTetO)_gRNA_handle_RPR1t
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(383..445)
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     misc_RNA        complement(458..533)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    625..643
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gggtaccaaa gaacaacaag agttccaaaa agtagtggaa caaaagcaaa tgaaggattt
       61 catgcgtttg tactctaatc tggtagaaag atgtttcaca gactgtgtca atgacttcac
      121 aacatcaaag ctaaccaata aggaacaaac atgcatcatg aagtgctcag aaaagttctt
      181 gaagcatagc gaacgtgtag ggcagcgttt ccaagaacaa aacgctgcct tgggacaagg
      241 cttgggccga taaggtgtac tggcgtatat atatctaatt atgtatctct ggtgtagccc
      301 atttttagca tgtaaatata aagagaaacc atatctaatc taaccaaatc caaacaaaat
      361 tcaatagtta ctatcgcttt tttctttctg tatcgcaaat aagtgaaaat taaaaaagaa
      421 agattaaatt ggaagttgga tatggctcga gaaaaaagca ccgactcggt gccacttttt
      481 caagttgata acggactagc cttattttaa cttgctattt ctagctctaa aacactgtat
      541 gtacatacag taaaagcttc tgccaatcgc agctcccaga gtttcgttcc cagccgcgag
      601 caccacagcg taccatgtgc gccatctcta tcactgatag ggacaatcga aacgaaaaga
      661 aacagactat aggggagtat agagacagcc gg
//
