LOCUS       sequence-106450-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Plasma Membrane, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             53..760
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag
       61 caagggcgag gagaccacaa tgggcgtaat caagcccgac atgaagatca agctgaagat
      121 ggagggcaac gtgaatggcc acgccttcgt gatcgagggc gagggcgagg gcaagcccta
      181 cgacggcacc aacaccatca acctggaggt gaaggaggga gcccccctgc ccttctccta
      241 cgacattctg accaccgcgt tcgcctacgg caacagggcc ttcaccaagt accccgacga
      301 catccccaac tacttcaagc agtccttccc cgagggctac tcttgggagc gcaccatgac
      361 cttcgaggac aagggcatcg tgaaggtgaa gtccgacatc tccatggagg aggactcctt
      421 catctacgag atacacctca agggcgagaa cttccccccc aacggccccg tgatgcagaa
      481 gaagaccacc ggctgggacg cctccaccga gaggatgtac gtgcgcgacg gcgtgctgaa
      541 gggcgacgtc aagcacaagc tgctgctgga gggcggcggc caccaccgcg ttgacttcaa
      601 gaccatctac agggccaaga aggcggtgaa gctgcccgac tatcactttg tggaccaccg
      661 catcgagatc ctgaaccacg acaaggacta caacaaggtg accgtttacg agagcgccgt
      721 ggcccgcaac tccaccgacg gcatggacga gctgtacaag tccggactca gatctaagct
      781 gaaccctcct gatgagagtg gccccggctg catgagctgc aagtgtgtgc tctcctgaga
      841 attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagataa
//
