LOCUS       sequence-106465-         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Lin TY, Voronovsky A, Raabe M, Urlaub H, Sander B, Golas MM
  TITLE     Dual tagging as an approach to isolate endogenous chromatin 
            remodeling complexes from Saccharomyces cerevisiae.
  JOURNAL   Biochim Biophys Acta. 2015 Mar;1854(3):198-208. doi: 
            10.1016/j.bbapap.2014.11.009. Epub 2014 Dec 5.
  PUBMED    25486077
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbapap.2014.11.009"; journalName: "Biochim Biophys Acta";
            date: "2015-03"; volume: "1854"; issue: "3"; pages: "198-208"
COMMENT     Sequence Label: pGS1044
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..85
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      111..298
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(166..190)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gctttctgct tctggttctg actacaaaga ccatgacggt gattataaag atcatgacat
       61 cgattacaag gatgacgatg acaagtaagt cgaccgccac ttctaaataa gcgaatttct
      121 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
      181 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
      241 tcaggttgct ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca
      301 gatccgctag ggataacagg gtaatataga tctgtttagc ttgcctcgtc cccgccgggt
      361 cacccggcca gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc
      421 tcaggggcat gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca
      481 tttgcatcca tacattttga tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg
      541 cagacctgcg agcagggaaa cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg
      601 cccctgtaga gaaatataaa aggttaggat ttgccactga
//
