LOCUS       sequence-106467-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a 
            LEU2 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Lin TY, Voronovsky A, Raabe M, Urlaub H, Sander B, Golas MM
  TITLE     Dual tagging as an approach to isolate endogenous chromatin 
            remodeling complexes from Saccharomyces cerevisiae.
  JOURNAL   Biochim Biophys Acta. 2015 Mar;1854(3):198-208. doi: 
            10.1016/j.bbapap.2014.11.009. Epub 2014 Dec 5.
  PUBMED    25486077
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbapap.2014.11.009"; journalName: "Biochim Biophys Acta";
            date: "2015-03"; volume: "1854"; issue: "3"; pages: "198-208"
COMMENT     Sequence Label: pGS1034
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..96
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             112..177
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             205..225
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             265..438
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             439..612
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     primer_bind     complement(723..747)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 cttggttctg gttctggtaa aagaagatgg aagaagaatt ttattgctgt ttctgctgct
       61 aatagattta agaaaatttc ttcttctggt gctttgtctg cttctggttc tgactacaaa
      121 gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga tgacaaggat
      181 tatgatattc caactactgc tagcgagaat ttgtattttc agggtgagct caaaaccgcg
      241 gctcttgcgc aacacgatga agccgtggac aacaaattca acaaagaaca acaaaacgcg
      301 ttctatgaga tcttacattt acctaactta aacgaagaac aacgaaacgc cttcatccaa
      361 agtttaaaag atgacccaag ccaaagcgct aaccttttag cagaagctaa aaagctaaat
      421 gatgctcagg cgccgaaagt agacaacaaa ttcaacaaag aacaacaaaa cgcgttctat
      481 gagatcttac atttacctaa cttaaacgaa gaacaacgaa acgccttcat ccaaagttta
      541 aaagatgacc caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatggtgct
      601 caggcgccga aagtagacgc gaattccgcg gggaagtcaa cctgagtcga ccgccacttc
      661 taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat
      721 aagtgtatac aaattttaaa gtgactctta
//
