LOCUS       sequence-106468-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal Strep tag II followed by TRP1 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1011
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttacgccnag cttgcatgcc gcacggtcgg agcgagggcc catgtttgat acatgattgc
       61 atggtatcac tacacacatc ccatataccc cggtatctct cactgaacaa ataataatgg
      121 tattttatgt ataaactcaa atcctttatt ctttattttt gtataatagt aatatcaatt
      181 ttaatataag gttatttcat tgaaaaattg aactatcaaa catcagttta ctaatcattt
      241 acttgggtac tctgagcgag ctaccctctt gcgaaatcta ctgtgatgct cgttaaagtg
      301 tgtggtttgc aaaccgttga agctgcaaag actgctgtgg atgatggtgc tgattactta
      361 ggtatcattt gtgttcccgg taggaaaaga accattgact catctgttgc gaaaggtatt
      421 tcaactgcag ttcaccaaca agagaacgtg aaaggtacta aactagtcgg ggtgtttaga
      481 aatcagtccg ttgatgatgt ccttcaactg taccacgaat ataatctaga tgtgatacaa
      541 ttacatggag atgaagatat taaagaatac agatctttga tcccatcttc aattccaatc
      601 attaagaggt tccagttccc acaggattgt gaattactac tggacctgta tgaacacgta
      661 gacaatgtgc tgacgttgtt cgattctggt gaaggtggca ctggtgagaa attgaattgg
      721 agtgcaattt ccagttggtc tgcaagtcat cccgagataa aattcattat cgctggtgga
      781 ttgaatcctg ataacgtttc tgttgccatt aatatgttac caaatgcgat cggtgtcgat
      841 gtaagtggag gagtagagac tgatggtatc aaggatttag aaaaggtaaa gctattcatc
      901 cagcaggcct ctcaatagag gaaaaaacgt atctttcttc aattacacat acgtatatat
      961 aaatt
//
