LOCUS       sequence-106470-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal Strep tag II followed by TRP1 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1011
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..44
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(79..95)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(79..95)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 agctcggtac cggtagtgct tggagtcatc cacaattcga aaaataaaag cttgatatcg
       61 aattcctgca gcccggggga tccactagtt ctagagcggc cgcggaacga tcattcacta
      121 tatatatatc aatttatata tacgtatgtg taattgaaga aagatacgtt ttttcctcta
      181 ttgagaggcc tgctggatga atagctttac cttttctaaa tccttgatac catcagtctc
      241 tactcctcca cttacatcga caccgatcgc atttggtaac atattaatgg caacagaaac
      301 gttatcagga ttcaatccac cagcgataat gaattttatc tcgggatgac ttgcagacca
      361 actggaaatt gcactccaat tcaatttctc accagtgcca ccttcaccag aatcgaacaa
      421 cgtcagcaca ttgtctacgt gttcatacag gtccagtagt aattcacaat cctgtgggaa
      481 ctggaacctc ttaatgattg gaattgaaga tgggatcaaa gatctgtatt ctttaatatc
      541 ttcatctcca tgtaattgta tcacatctag attatattcg tggtacagtt gaaggacatc
      601 atcaacggac tgatttctaa acaccccgac tagtttagta cctttcacgt tctcttgttg
      661 gtgaactgca gttgaaatac ctttcgcaac agatgagtca atggttcttt tcctaccggg
      721 aacacaaatg atacctaagt aatcagcacc atcatccaca gcagtctttg cagcttcaac
      781 ggtttgcaaa ccacacactt taacgagcat cacagtagat ttcgcaagag ggtagctcgc
      841 tcagagtacc caagtaaatg attagtaaac tgatgtttga tagttcaatt tttcaatgaa
      901 ataaccttat attaaaattg atattactat tatacaaaaa taaagaataa aggatttgag
      961 tttatacata aaata
//
