LOCUS       sequence-106504-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To fuse your protein of interest to the N-terminus of BirA(R118G) 
            and use in proximity-dependent biotin identification (BioID); HA 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Roux KJ, Kim DI, Raida M, Burke B
  TITLE     A promiscuous biotin ligase fusion protein identifies proximal and 
            interacting proteins in mammalian cells.
  JOURNAL   J Cell Biol. 2012 Mar 12.
  PUBMED    22412018
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2012 Mar 12."
COMMENT     Sequence Label: pcDNA3.1 MCS-BirA(R118G)-HA
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atccggtaca tcgtaagggt aagccttctc tgcgcttctc agggagattt ctccgcccat
       61 ccagggcttg atgattccgt cctgctccag cagcagagct ccctgcttgt cgattcctct
      121 ggagatgccg aagatttcct tgtcgccgat gatcagcttc acaggtctgt tgatgaagtt
      181 gtccagcttc tcccatctgc tcaggtaagg agccagtccc tcctgctcga acagctccag
      241 agcggctctc agctctctga tcagcatggc ggccagggtg ttccggtcca ggttgatgcc
      301 agcctcctgc agggtgatcc agccctggtt caccacgctc tcctccactc ttctcatggc
      361 catgttgatt ccggctccga tcacgatctg agcggcatcg cctgtcttgc ctgtcagctc
      421 caccaggatg ccagccagct tgcggtcctg gaggtacagg tcattaggcc acttcactct
      481 caccttgtcg gctcccagct ttctcagcac ctcggccatc acgattccga tcaccagact
      541 cagtccgatg gcagcagcag gtccctgctc cagtctccag aacatggaca ggtacaggtt
      601 ggctccgaat gggctgaacc acttccgtcc tctgcctcct ctgccagcct gctggtactc
      661 ggcgatgcag gcgtcgccgg acttcagctc tccgattctg tccagcaggt actgattggt
      721 ggagtcgatc acaggcagca cggccacgct tccgccatcc agctgtccca ggatctgctt
      781 ggcgttcagc agctggatag gctcaggcag gctgtagccc ttgccaggca cggtgaacac
      841 gtccactccc cagtct
//
