LOCUS       sequence-106520-        3710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast ORF Rrp8 in the Gateway entry vector pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: pDONR221 Rrp8
FEATURES             Location/Qualifiers
     source          1..3710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1951..1970)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1952..1970)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1975..1991)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1975..1991)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2093..2112)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2104..2913
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2171..2190)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3060..3648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3549..3568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agatggcctt atttaacgta gaaggttggt ctattaagac aaaaaccgtc gcttttgata
      721 acaagactaa taagtcttca aaagataaaa aaaaaaataa tagaaaaaat ggcaaactta
      781 caagagaaca gaagttgaaa gaagagacag aggctgagtt gaaggaacaa gtggaagaca
      841 ttccttccga aggatcagta gccaaagaca tcccaaaaaa aaaccaagag aaaagtgatc
      901 aaaatgaaac gagcaagaaa cgcaagcacg atgaagaagc ccctctgatg caagtaaaag
      961 aaaatattga aaaacctaca aagaagcaac taaccccttt acaacaaaag atgatggcta
     1021 aactgactgg ttctagattt agatggatca atgaacaact gtatacaatt agctctgatg
     1081 aagctttgaa attaataaaa gaacaaccac aattatttga cgaatatcat gatggtttta
     1141 gatcacaagt gcaagcatgg ccggaaaatc cagttgatgt ttttgttgac caaattcgtt
     1201 atagatgcat gaaacctgtg aatgctccag gtgggttacc aggtcttaag gatagtaaag
     1261 aaatagttat tgctgatatg gggtgtggtg aagctcaatt agcattagaa atcaacaatt
     1321 ttttcaaaaa ttacaataag aaagcgaaga aatatttgaa aagacgccat aaagtccaca
     1381 gttttgattt gaagaaagct aacgaaagaa taactgtggc agatattaga aatgtgccgc
     1441 taccagatga gtcctgtact atagtggtct tctgcctggc tctaatgggt acaaatttcc
     1501 tcgatttcat aaaagaagct tataggattt tagcgccaag gggtgaatta tggatcgcag
     1561 aaattaaatc aaggtttagt gacggcaaag gtaatgaatt tgtagacgct ttgaagctga
     1621 tgggattttt tcacaaaaag accttcgacg agaataagat gtttacaaga ttcgaatttt
     1681 tcaagccacc tgcggagatt attgaagaga gaaggcagaa attggaaaga agacaaaagt
     1741 ttattgaagt tgaaactgag aaggaagaat tagaaaagaa aagacggaaa attgccgaag
     1801 gaaaatggct cttgaagccc tgtatttata aaagaagaag ctttcttgta caaagttggc
     1861 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1921 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     1981 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     2041 aaaataatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2101 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2161 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2221 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2281 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2341 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2401 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2461 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2521 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2581 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2641 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     2701 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     2761 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     2821 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     2881 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     2941 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     3001 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3061 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3121 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3181 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3241 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3301 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3361 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3421 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3481 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3541 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3601 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3661 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
