LOCUS       sequence-106529-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 355, Emission: 424.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Sirius-H2B-6
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(134..385)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKAHFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLESVTAAGITLGMDELYK"
     primer_bind     525..544
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     784..805
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      847..856
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ataacagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
       61 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag
      121 tcgcggccgc ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacgg
      181 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactgga
      241 cgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
      301 ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc ttgaagtggg
      361 ccttgatgcc gttcttctgc ttgtcggcgg tgatatatac gtttgagctt atcccgttgt
      421 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgaagccc ttcagctcga
      481 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      541 cgtccttgaa gaagatggta cgctcctgga cgtagccatc gggcatggcg gacttgaaga
      601 agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca cagcacgccg aattgcaggg
      661 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg agcttcaggg
      721 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ctgtggccgt
      781 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
      841 tgctcaccat ggtggcgacc ggtggatcct t
//
