LOCUS       sequence-106562-        6926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is designed as a bacterial ethanol sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6926)
  TITLE     Salis Lab Plasmids
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: rpoS700-RFP
FEATURES             Location/Qualifiers
     source          1..6926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(130..147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(264..809)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(344..363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     885..904
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             2598..3269
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="SSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLK
                     VTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVT
                     VTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRL
                     KLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTG
                     A"
     promoter        3344..3378
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     terminator      4213..4299
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(4382..4399)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4552..4638
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4730..4757
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4776..4867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4868..4936,4937..5728)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5086..5105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5770..6225
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5857..5876)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc
       61 atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata
      121 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat
      181 ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta acagggaagt
      241 gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa
      301 atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      361 ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
      421 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
      481 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc
      541 cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc
      601 cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg
      661 aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg
      721 tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag
      781 agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt
      841 tctaggatca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      901 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      961 aggatctgct catgtttgac agcttatcat cgatgcataa tgtgcctgtc aaatggacga
     1021 agcagggatt ctgcaaaccc tatgctactc cctcgagccg gatccaatat tcaggcacca
     1081 tacgcgctga acgttggtca gaccttgcag gtgggtaatg cttccggtac gccaatcact
     1141 ggcggaaatg ccattaccca ggccgacgca gcagagcaag gagttgtgat caagcctgca
     1201 caaaattcca ccgttgctgt tgcgtcgcaa ccgacaatta cgtattctga gtcttcgggt
     1261 gaacagagtg ctaacaaaat gttgccgaac aacaagccaa ctgcgaccac ggtcacagcg
     1321 cctgtaacgg taccaacagc aagcacaacc gagccgactg tcagcagtac atcaaccagt
     1381 acgcctatct ccacctggcg ctggccgact gagggcaaag tgatcgaaac ctttggcgct
     1441 tctgaggggg gcaacaaggg gattgatatc gcaggcagca aaggacaggc aattatcgcg
     1501 accgcagatg gccgcgttgt ttatgctggt aacgcgctgc gcggctacgg taatctgatt
     1561 atcatcaaac ataatgatga ttacctgagt gcctacgccc ataacgacac aatgctggtc
     1621 cgggaacaac aagaagttaa ggcggggcaa aaaatagcga ccatgggtag caccggaacc
     1681 agttcaacac gcttgcattt tgaaattcgt tacaagggga aatccgtaaa cccgctgcgt
     1741 tatttgccgc agcgataaat cggcggaacc aggcttttgc ttgaatgttc cgtcaaggga
     1801 tcacgggtag gagccacctt atgagtcaga atacgctgaa agttcatgat ttaaatgaag
     1861 atgcggaatt tgatgagaac ggagttgagg tttttgacga aaaggcctta gtagaacagg
     1921 aacccagtga taacgatttg gccgaagagg aactgttatc gcagggagcc acacagcgtg
     1981 tgttggacgc gactcagctt taccttggtg agattggtta ttcaccactg ttaacggccg
     2041 aagaagaagt ttattttgcg cgtcgcgcac tgcgtggaga tgtcgcctct cgccgccgga
     2101 tgatcgagag taacttgcgt ctggtggtaa aaattgcccg ccgttatggc aatcgtggtc
     2161 tggcgttgct ggaccttatc gaagagggca acctggggct gatccgcgcg gtagagaagt
     2221 ttgacccgga acgtggtttc cgcttctcaa catacgcaac ctggtggatt cgccagacga
     2281 ttgaacgggc gattatgaac caaacccgta ctattcgttt gccgattcac atcgtaaagg
     2341 agctgaacgt ttacctgcga accgcacgtg agttgtccca taagctggac catgaaccaa
     2401 gtgcggaaga gatcgcagag caactggata agccagttga tgacgtcagc cgtatgcttc
     2461 gtcttaacga gcgcattacc tcggtagaca ccccgctggg tggtgattcc gaaaaagcgt
     2521 tgctggacat cctggccgat gaaaaagaga acggtccgga agataccacg caagatgacg
     2581 agaattctaa aaatctgagc tctgaagacg ttatcaaaga gttcatgcgt ttcaaagttc
     2641 gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc
     2701 cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg
     2761 cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg
     2821 ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta
     2881 tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg
     2941 agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc
     3001 agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc
     3061 tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag
     3121 ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca
     3181 tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg
     3241 ctgaaggtcg tcactccacc ggtgcttaat aaggccggcc gtgctagtgt agaaatagcc
     3301 gccagttccg ctggcggcat tttaactttc tttagtacta gtattgacag ctagctcagt
     3361 cctaggtact gtgctagcac cccaatcgac gtcatactag aatcgggtca cggaggtaca
     3421 ataataatgc gtaaactcga ggaacttttc acgggggtcg tcccaattct ggtagaatta
     3481 gacggtgatg taaacgggca caagtttagc gtcagcggag agggcgaggg ggacgccacc
     3541 tacggtaaac ttacccttaa gtttatttgc acgacgggca aattacccgt tccgtggcca
     3601 acgctcgtca ctactttcgg ctacggcgtt cagtgcttcg cgagatatcc ggatcacatg
     3661 aagcagcacg atttcttcaa gtcggccatg ccagagggtt acgttcagga gagaactata
     3721 tttttcaagg acgacggcaa ttacaagaca cgcgccgaag tcaagttcga aggcgatacc
     3781 ctggttaacc gtatcgagtt aaaaggtatc gattttaaag aagacggaaa cattctcggt
     3841 cacaaactcg aatacaacta taacagccat aatgtctaca ttatggctga caaacagaag
     3901 aacggcatta aggttaactt caaaattcgc cacaacatcg aagacggtag cgtccaacta
     3961 gcagaccatt atcaacaaaa tactcctatc ggcgacggcc cagtcctttt accagacaac
     4021 cattacctta gcacacaaag cgcccttagc aaagatccca acgagaagcg cgaccacatg
     4081 gtccttctcg aatttgtcac agcggcaggg atcacccacg gcatggacga actctacaaa
     4141 taataattaa ttaaaaaaaa aaaccccgcc cctgacaggg cggggttttt ttttctaata
     4201 tgcggccgcc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     4261 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gagcatgcgc
     4321 gttcggctgc ggcgagcggt atcaggcttg gctgttttgg cggatgagag aagattttca
     4381 gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg
     4441 gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg
     4501 ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa
     4561 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     4621 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
     4681 gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc
     4741 ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc
     4801 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4861 gaagactatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4921 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4981 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5041 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5101 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5161 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5221 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5281 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5341 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5401 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5461 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5521 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5581 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5641 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5701 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5761 gattgattta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5821 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5881 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5941 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     6001 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6061 ttaatagtgg actcttgttc caaacttgaa caacactcaa ccctatctcg ggctattctt
     6121 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6181 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttaaaag gatctaggtg
     6241 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6301 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6361 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6421 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6481 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6541 tacctcgctc tgctaatcct gttaccagtc aggcatttga gaagcacacg gtcacactgc
     6601 ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct
     6661 gccctgaacc gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt
     6721 atcacttatt caggcgtagc accaggcgtt taagggcacc aataactgcc ttaaaaaaat
     6781 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     6841 tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     6901 ccttgcgtat aatatttgcc catggc
//
