LOCUS       sequence-106563-        6356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is designed as a bacterial stationary phase sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6356)
  TITLE     Salis Lab Plasmids
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: rpoS200-RFP
FEATURES             Location/Qualifiers
     source          1..6356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(130..147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(264..809)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(344..363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     885..904
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             2028..2699
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="SSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLK
                     VTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVT
                     VTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRL
                     KLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTG
                     A"
     promoter        2774..2808
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     terminator      3643..3729
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3812..3829)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3982..4068
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4160..4187
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4206..4297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4298..4366,4367..5158)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4516..4535)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5200..5655
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5287..5306)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc
       61 atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata
      121 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat
      181 ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta acagggaagt
      241 gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa
      301 atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      361 ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
      421 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
      481 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc
      541 cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc
      601 cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg
      661 aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg
      721 tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag
      781 agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt
      841 tctaggatca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      901 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      961 aggatctgct catgtttgac agcttatcat cgatgcataa tgtgcctgtc aaatggacga
     1021 agcagggatt ctgcaaaccc tatgctactc cctcgagccg gatccaatat tcaggcacca
     1081 tacgcgctga acgttggtca gaccttgcag gtgggtaatg cttccggtac gccaatcact
     1141 ggcggaaatg ccattaccca ggccgacgca gcagagcaag gagttgtgat caagcctgca
     1201 caaaattcca ccgttgctgt tgcgtcgcaa ccgacaatta cgtattctga gtcttcgggt
     1261 gaacagagtg ctaacaaaat gttgccgaac aacaagccaa ctgcgaccac ggtcacagcg
     1321 cctgtaacgg taccaacagc aagcacaacc gagccgactg tcagcagtac atcaaccagt
     1381 acgcctatct ccacctggcg ctggccgact gagggcaaag tgatcgaaac ctttggcgct
     1441 tctgaggggg gcaacaaggg gattgatatc gcaggcagca aaggacaggc aattatcgcg
     1501 accgcagatg gccgcgttgt ttatgctggt aacgcgctgc gcggctacgg taatctgatt
     1561 atcatcaaac ataatgatga ttacctgagt gcctacgccc ataacgacac aatgctggtc
     1621 cgggaacaac aagaagttaa ggcggggcaa aaaatagcga ccatgggtag caccggaacc
     1681 agttcaacac gcttgcattt tgaaattcgt tacaagggga aatccgtaaa cccgctgcgt
     1741 tatttgccgc agcgataaat cggcggaacc aggcttttgc ttgaatgttc cgtcaaggga
     1801 tcacgggtag gagccacctt atgagtcaga atacgctgaa agttcatgat ttaaatgaag
     1861 atgcggaatt tgatgagaac ggagttgagg tttttgacga aaaggcctta gtagaacagg
     1921 aacccagtga taacgatttg gccgaagagg aactgttatc gcagggagcc acacagcgtg
     1981 tgttggacgc gactcagctt taccttggtt tgaattctaa aaatctgagc tctgaagacg
     2041 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt
     2101 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga
     2161 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt
     2221 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct
     2281 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg
     2341 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta
     2401 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca
     2461 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
     2521 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
     2581 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
     2641 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
     2701 aaggccggcc gtgctagtgt agaaatagcc gccagttccg ctggcggcat tttaactttc
     2761 tttagtacta gtattgacag ctagctcagt cctaggtact gtgctagcac cccaatcgac
     2821 gtcatactag aatcgggtca cggaggtaca ataataatgc gtaaactcga ggaacttttc
     2881 acgggggtcg tcccaattct ggtagaatta gacggtgatg taaacgggca caagtttagc
     2941 gtcagcggag agggcgaggg ggacgccacc tacggtaaac ttacccttaa gtttatttgc
     3001 acgacgggca aattacccgt tccgtggcca acgctcgtca ctactttcgg ctacggcgtt
     3061 cagtgcttcg cgagatatcc ggatcacatg aagcagcacg atttcttcaa gtcggccatg
     3121 ccagagggtt acgttcagga gagaactata tttttcaagg acgacggcaa ttacaagaca
     3181 cgcgccgaag tcaagttcga aggcgatacc ctggttaacc gtatcgagtt aaaaggtatc
     3241 gattttaaag aagacggaaa cattctcggt cacaaactcg aatacaacta taacagccat
     3301 aatgtctaca ttatggctga caaacagaag aacggcatta aggttaactt caaaattcgc
     3361 cacaacatcg aagacggtag cgtccaacta gcagaccatt atcaacaaaa tactcctatc
     3421 ggcgacggcc cagtcctttt accagacaac cattacctta gcacacaaag cgcccttagc
     3481 aaagatccca acgagaagcg cgaccacatg gtccttctcg aatttgtcac agcggcaggg
     3541 atcacccacg gcatggacga actctacaaa taataattaa ttaaaaaaaa aaaccccgcc
     3601 cctgacaggg cggggttttt ttttctaata tgcggccgcc atcaaataaa acgaaaggct
     3661 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     3721 aggacaaatc cgccgcccta gagcatgcgc gttcggctgc ggcgagcggt atcaggcttg
     3781 gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa
     3841 gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca
     3901 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga
     3961 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     4021 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     4081 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     4141 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
     4201 actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4261 ctgataaatg cttcaataat attgaaaaag gaagactatg agtattcaac atttccgtgt
     4321 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4381 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4441 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4501 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     4561 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4621 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4681 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4741 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4801 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
     4861 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4921 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4981 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5041 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5101 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5161 gtcagaccaa gtttactcat atatacttta gattgattta cgcgccctgt agcggcgcat
     5221 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5281 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5341 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5401 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5461 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaacttgaa
     5521 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     5581 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     5641 taacgtttac aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5701 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5761 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5821 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5881 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     5941 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtc
     6001 aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc
     6061 aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg tcgaatttgc
     6121 tttcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc accaggcgtt
     6181 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
     6241 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacagacggc atgatgaacc
     6301 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggc
//
