LOCUS       sequence-106565-        6331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is designed as a bacterial cell division sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6331)
  TITLE     Salis Lab Plasmids
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ftsZ-RFP
FEATURES             Location/Qualifiers
     source          1..6331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(132..149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(266..811)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(346..365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     887..906
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             2005..2676
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="SSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLK
                     VTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVT
                     VTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRL
                     KLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTG
                     A"
     promoter        2751..2785
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     terminator      3620..3706
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3789..3806)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3959..4045
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4137..4164
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4183..4274
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4275..4343,4344..5135)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4493..4512)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5177..5632
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5264..5283)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
       61 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
      121 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
      181 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
      241 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
      301 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
      361 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
      421 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
      481 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
      541 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
      601 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
      661 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
      721 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
      781 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata
      841 tttctaggat catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
      901 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
      961 agaggatctg ctcatgtttg acagcttatc atcgatgcat aatgtgcctg tcaaatggac
     1021 gaagcaggga ttctgcaaac cctatgctac tccctcgagc cggatccact ggcatcaagt
     1081 tattcggtat tgacggaaga tgaacgtgaa ctgggtgtct gcgtcgtcga tatcggtggt
     1141 ggtacaatgg atatcgccgt ttataccggt ggggcattgc gccacactaa ggtaattcct
     1201 tatgctggca atgtcgtgac cagtgatatc gcttacgcct ttggcacgcc gccaagcgac
     1261 gccgaagcga ttaaagttcg ccacggttgt gcgctgggtt ccatcgttgg aaaagatgag
     1321 agcgtggaag tgccgagcgt aggtggtcgt ccgccacgga gtctgcaacg tcagacactg
     1381 gcagaggtga tcgagccgcg ctataccgag ctgctcaacc tggtcaacga agagatattg
     1441 cagttgcagg aaaagcttcg ccaacaaggg gttaaacatc acctggcggc aggcattgta
     1501 ttaaccggtg gcgcagcgca gatcgaaggt cttgcagcct gtgctcagcg cgtgtttcat
     1561 acgcaagtgc gtatcggcgc gccgctgaac attaccggtt taacggatta tgctcaggag
     1621 ccgtattatt cgacggcggt gggattgctt cactatggga aagagtcaca tcttaacggt
     1681 gaagctgaag tagaaaaacg tgttacagca tcagttggct cgtggatcaa gcgactcaat
     1741 agttggctgc gaaaagagtt ttaattttta tgaggccgac gatgattacg gcctcaggcg
     1801 acaggcacaa atcggagaga aactatgttt gaaccaatgg aacttaccaa tgacgcggtg
     1861 attaaagtca tcggcgtcgg cggcggcggc ggtaatgctg ttgaacacat ggtgcgcgag
     1921 cgcattgaag gtgttgaatt cttcgcggta aataccgatg cacaagcgct gcgtaaaaca
     1981 gcggttggac agacgattca aaagagctct gaagacgtta tcaaagagtt catgcgtttc
     2041 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
     2101 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
     2161 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
     2221 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
     2281 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
     2341 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
     2401 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
     2461 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
     2521 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
     2581 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
     2641 gaacgtgctg aaggtcgtca ctccaccggt gcttaataag gccggccgtg ctagtgtaga
     2701 aatagccgcc agttccgctg gcggcatttt aactttcttt agtactagta ttgacagcta
     2761 gctcagtcct aggtactgtg ctagcacccc aatcgacgtc atactagaat cgggtcacgg
     2821 aggtacaata ataatgcgta aactcgagga acttttcacg ggggtcgtcc caattctggt
     2881 agaattagac ggtgatgtaa acgggcacaa gtttagcgtc agcggagagg gcgaggggga
     2941 cgccacctac ggtaaactta cccttaagtt tatttgcacg acgggcaaat tacccgttcc
     3001 gtggccaacg ctcgtcacta ctttcggcta cggcgttcag tgcttcgcga gatatccgga
     3061 tcacatgaag cagcacgatt tcttcaagtc ggccatgcca gagggttacg ttcaggagag
     3121 aactatattt ttcaaggacg acggcaatta caagacacgc gccgaagtca agttcgaagg
     3181 cgataccctg gttaaccgta tcgagttaaa aggtatcgat tttaaagaag acggaaacat
     3241 tctcggtcac aaactcgaat acaactataa cagccataat gtctacatta tggctgacaa
     3301 acagaagaac ggcattaagg ttaacttcaa aattcgccac aacatcgaag acggtagcgt
     3361 ccaactagca gaccattatc aacaaaatac tcctatcggc gacggcccag tccttttacc
     3421 agacaaccat taccttagca cacaaagcgc ccttagcaaa gatcccaacg agaagcgcga
     3481 ccacatggtc cttctcgaat ttgtcacagc ggcagggatc acccacggca tggacgaact
     3541 ctacaaataa taattaatta aaaaaaaaaa ccccgcccct gacagggcgg ggtttttttt
     3601 tctaatatgc ggccgccatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     3661 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagag
     3721 catgcgcgtt cggctgcggc gagcggtatc aggcttggct gttttggcgg atgagagaag
     3781 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg
     3841 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
     3901 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca
     3961 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     4021 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
     4081 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa
     4141 ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta tttttctaaa
     4201 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4261 gaaaaaggaa gactatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4321 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4381 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4441 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4501 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4561 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4621 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4681 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4741 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4801 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     4861 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4921 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4981 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5041 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5101 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5161 tactttagat tgatttacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5221 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5281 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5341 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     5401 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5461 acgttcttta atagtggact cttgttccaa acttgaacaa cactcaaccc tatctcgggc
     5521 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5581 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttaaaaggat
     5641 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5701 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5761 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5821 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5881 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5941 gcctacatac ctcgctctgc taatcctgtt accagtcagg catttgagaa gcacacggtc
     6001 acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa
     6061 cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg ccattcatcc
     6121 gcttattatc acttattcag gcgtagcacc aggcgtttaa gggcaccaat aactgcctta
     6181 aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     6241 gccgacatgg aagccatcac agacggcatg atgaacctga atcgccagcg gcatcagcac
     6301 cttgtcgcct tgcgtataat atttgcccat g
//
