LOCUS       sequence-106567-        5981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is designed as a bacterial oxidative stress sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5981)
  TITLE     Salis Lab Plasmids
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: katG-RFP
FEATURES             Location/Qualifiers
     source          1..5981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(132..149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(266..811)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(346..365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     887..906
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     RBS             1635..1643
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1649..2326
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     promoter        2401..2435
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     terminator      3270..3356
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3439..3456)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3609..3695
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3787..3814
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3833..3924
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3925..3993,3994..4785)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4143..4162)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4827..5282
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4914..4933)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
       61 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
      121 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
      181 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
      241 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
      301 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
      361 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
      421 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
      481 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
      541 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
      601 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
      661 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
      721 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
      781 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata
      841 tttctaggat catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
      901 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
      961 agaggatctg ctcatgtttg acagcttatc atcgatgcat aatgtgcctg tcaaatggac
     1021 gaagcaggga ttctgcaaac cctatgctac tccctcgagc cggatcccct ggtttataaa
     1081 tagtgtggct tttgtgaaaa tcacacagtg atcacaaatt ttaaacagag cacaaaatgc
     1141 tgcctcgaaa tgagggcggg aaaataaggt tatcagcctt gttttctccc tcattacttg
     1201 aaggatatga agctaaaacc cttttttata aagcatttgt ccgaattcgg acataatcaa
     1261 aaaagcttaa ttaagatcaa tttgatctac atctctttaa ccaacaatat gtaagatctc
     1321 aactatcgca tccgtggatt aattcaatta taacttctct ctaacgctgt gtatcgtaac
     1381 ggtaacactg tagaggggag cacattgatg agcacgtcag acgatatcca taacaccaca
     1441 gccactggca aatgcccgtt ccatcagggc ggtcacgacc agagtgcggg ggcgggcaca
     1501 accactcgcg actggtggcc aaatcaactt cgtgttgacc tgttaaacca acattctaat
     1561 cgttctaacc cactgggtga ggactttgac taccgcaaag aattcagcaa attagaataa
     1621 tctagaaaaa aaaataagga ggtaaaaaat ggcgagctct gaagacgtta tcaaagagtt
     1681 catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg
     1741 tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg
     1801 tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc
     1861 ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt
     1921 caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc
     1981 ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc
     2041 cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta
     2101 cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg
     2161 tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg
     2221 tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt
     2281 tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataag gccggccgtg
     2341 ctagtgtaga aatagccgcc agttccgctg gcggcatttt aactttcttt agtactagta
     2401 ttgacagcta gctcagtcct aggtactgtg ctagcacccc aatcgacgtc atactagaat
     2461 cgggtcacgg aggtacaata ataatgcgta aactcgagga acttttcacg ggggtcgtcc
     2521 caattctggt agaattagac ggtgatgtaa acgggcacaa gtttagcgtc agcggagagg
     2581 gcgaggggga cgccacctac ggtaaactta cccttaagtt tatttgcacg acgggcaaat
     2641 tacccgttcc gtggccaacg ctcgtcacta ctttcggcta cggcgttcag tgcttcgcga
     2701 gatatccgga tcacatgaag cagcacgatt tcttcaagtc ggccatgcca gagggttacg
     2761 ttcaggagag aactatattt ttcaaggacg acggcaatta caagacacgc gccgaagtca
     2821 agttcgaagg cgataccctg gttaaccgta tcgagttaaa aggtatcgat tttaaagaag
     2881 acggaaacat tctcggtcac aaactcgaat acaactataa cagccataat gtctacatta
     2941 tggctgacaa acagaagaac ggcattaagg ttaacttcaa aattcgccac aacatcgaag
     3001 acggtagcgt ccaactagca gaccattatc aacaaaatac tcctatcggc gacggcccag
     3061 tccttttacc agacaaccat taccttagca cacaaagcgc ccttagcaaa gatcccaacg
     3121 agaagcgcga ccacatggtc cttctcgaat ttgtcacagc ggcagggatc acccacggca
     3181 tggacgaact ctacaaataa taattaatta aaaaaaaaaa ccccgcccct gacagggcgg
     3241 ggtttttttt tctaatatgc ggccgccatc aaataaaacg aaaggctcag tcgaaagact
     3301 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     3361 cgccctagag catgcgcgtt cggctgcggc gagcggtatc aggcttggct gttttggcgg
     3421 atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
     3481 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
     3541 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
     3601 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3661 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     3721 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     3781 ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta
     3841 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3901 caataatatt gaaaaaggaa gactatgagt attcaacatt tccgtgtcgc ccttattccc
     3961 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4021 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4081 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4141 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     4201 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4261 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4321 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4381 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4441 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     4501 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4561 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4621 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4681 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4741 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4801 tactcatata tactttagat tgatttacgc gccctgtagc ggcgcattaa gcgcggcggg
     4861 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4921 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4981 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5041 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5101 gttggagtcc acgttcttta atagtggact cttgttccaa acttgaacaa cactcaaccc
     5161 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5221 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     5281 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5341 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5401 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5461 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5521 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5581 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtcagg catttgagaa
     5641 gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc
     5701 ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg
     5761 ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa gggcaccaat
     5821 aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     5881 taagcattct gccgacatgg aagccatcac agacggcatg atgaacctga atcgccagcg
     5941 gcatcagcac cttgtcgcct tgcgtataat atttgcccat g
//
