LOCUS       sequence-106574-        2798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 
            gene, using a weak AEB3 promoter. It should be used when testing 
            AEB-DNT3 riboswitch to measure the non-specific DNT effect on mRFP1 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-No-aptamer-control -- AEB3mRFP1
FEATURES             Location/Qualifiers
     source          1..2798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        65..99
                     /label=BBa_J23116
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.16"
     RBS             114..122
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             128..805
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      850..921
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      937..964
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1006..1092
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1257..1845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1337..1356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1934..2028)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2049..2708)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2623..2642
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2709..2798,1..13))
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tacgtgccga tcaacgtctc attttcgcca gatatcctcg agcgcggaat tccctagggg
       61 atccttgaca gctagctcag tcctagggac tatgctagct ctagaaaaaa aaataaggag
      121 gtaaaaaatg gcgagctctg aagacgttat caaagagttc atgcgtttca aagttcgtat
      181 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta
      241 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg
      301 ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga
      361 catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa
      421 cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt
      481 catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa
      541 aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa
      601 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa
      661 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa
      721 actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga
      781 aggtcgtcac tccaccggtg cttaataagg ccggccgtgc tagtgtagat cgctactaga
      841 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      901 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      961 tgcgtttata caattgacta gtctgcagaa aagcggccgc ggcatcaaat aaaacgaaag
     1021 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     1081 agtaggacaa atccgccgcc ctagagcatg cgcgttcggc tgcggcgagc ggtatcagct
     1141 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1201 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1261 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1321 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1381 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1441 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1501 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1561 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1621 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1681 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1741 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1801 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1861 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1921 cccgggtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
     1981 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcggtaccg
     2041 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     2101 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     2161 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     2221 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     2281 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     2341 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     2401 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     2461 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     2521 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     2581 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     2641 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     2701 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     2761 agtgatctta tttcattatg gtgaaagttg gaacctct
//
