LOCUS       sequence-106575-        2906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This pFTV1 vector containsa 2,4,dinitrotoluene (DNT) binding 
            riboswitch, controlling the translation initaition rate of mRFP1 
            gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-DNT3
FEATURES             Location/Qualifiers
     source          1..2906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        65..99
                     /label=BBa_J23116
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.16"
     CDS             236..913
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      958..1029
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1045..1072
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1114..1200
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1365..1953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1445..1464)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2042..2136)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2157..2816)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2731..2750
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2817..2906,1..13))
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tacgtgccga tcaacgtctc attttcgcca gatatcctcg agcgcggaat tccctagggg
       61 atccttgaca gctagctcag tcctagggac tatgctagct ctagaggcta aattagcact
      121 actttccagc tcggtaccat aacacaagtg gtagactatt ctctggtacg tgcgcccccg
      181 gccgtattac gggagcacgc cggctaaggg tcgtagcacc tagaggaggt caaatatggc
      241 gagctctgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
      301 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
      361 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
      421 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
      481 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
      541 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
      601 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
      661 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
      721 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
      781 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
      841 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
      901 caccggtgct taataaggcc ggccgtgcta gtgtagatcg ctactagagc caggcatcaa
      961 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1021 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttataca
     1081 attgactagt ctgcagaaaa gcggccgcgg catcaaataa aacgaaaggc tcagtcgaaa
     1141 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     1201 ccgccgccct agagcatgcg cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1261 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1321 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1381 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1441 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1501 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1561 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1621 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1681 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1741 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1801 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1861 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1921 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1981 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgcc cgggtgcttg
     2041 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     2101 gttctgaggt cattactgga tctatcaaca ggagtccaag cggtaccgat atcaaattac
     2161 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     2221 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     2281 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     2341 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
     2401 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     2461 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     2521 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     2581 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     2641 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     2701 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     2761 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     2821 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     2881 tcattatggt gaaagttgga acctct
//
