LOCUS       sequence-106576-        3643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This pFTV1 vector contains a no-aptamer control mRNA encoding 
            luciferase gene. It should be used when testing AEB-Dopa5, AEB-T4-2 
            riboswitches to measure their non-specific ligand effect on 
            luciferase expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-No-aptamer-controlluciferase
FEATURES             Location/Qualifiers
     source          1..3643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(71..76,77..92,93..99)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     RBS             119..127
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             145..1794
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(208..226)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      1851..1937
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2102..2690)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2182..2201)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2779..2873)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2894..3553)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3468..3487
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(3554..3643,1..13))
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tacgtgccga tcaacgtctc attttcgcca gatatcctcg agcgcggaat tccctagggg
       61 atccgagctg ttgacaatta atcatcggct cgtataatgt gtgggacgtc aaaaaaaata
      121 aggaggtaaa aaatggaagc tttcgaagac gccaaaaaca taaagaaagg cccggcgcca
      181 ttctatcctc tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac
      241 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac
      301 gcggaatact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat
      361 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg
      421 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa
      481 ttgctcaaca gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg
      541 caaaaaattt tgaacgtgca aaaaaaatta ccaataatcc agaaaattat tatcatggat
      601 tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct
      661 cccggtttta atgaatacga ttttgtacca gagtcctttg atcgtgacaa aacaattgca
      721 ctgataatga attcctctgg atctactggg ttacctaagg gtgtggccct tccgcataga
      781 actgcctgcg tcagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg
      841 gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc
      901 ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt
      961 ttacgatccc ttcaggatta caaaattcaa agtgcgttgc tagtaccaac cctattttca
     1021 ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct
     1081 tctgggggcg cacctctttc gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt
     1141 ccagggatac gacaaggata tgggctcact gagactacat cagctattct gattacaccc
     1201 gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt
     1261 gtggatctgg ataccgggaa aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga
     1321 ggacctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac
     1381 aaggatggat ggctacattc tggagacata gcttactggg acgaagacga acacttcttc
     1441 atagttgacc gcttgaagtc tttaattaaa tacaaaggat atcaggtggc ccccgctgaa
     1501 ttggaatcga tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtcttccc
     1561 gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg
     1621 acggaaaaag agatcgtgga ttacgtggcc agtcaagtaa caaccgcgaa aaagttgcgc
     1681 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga
     1741 aaaatcagag agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaaccgcac
     1801 accttactgg tgtgcggact agtccaactg cagaaaagcg gccgcggcat caaataaaac
     1861 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1921 tcctgagtag gacaaatccg ccgccctaga gcatgcgcgt tcggctgcgg cgagcggtat
     1981 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2041 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2101 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2161 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2221 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2281 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2341 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2401 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2461 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2521 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     2581 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2641 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2701 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2761 tcatgcccgg gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     2821 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcgg
     2881 taccgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     2941 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     3001 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     3061 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     3121 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     3181 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     3241 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     3301 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     3361 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     3421 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     3481 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     3541 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     3601 ccggtagtga tcttatttca ttatggtgaa agttggaacc tct
//
