LOCUS       sequence-106577-        3722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This pFTV1 vector contains a dopamine binding riboswitch, 
            controlling the translation initaition rate of luciferase gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-Dopa5
FEATURES             Location/Qualifiers
     source          1..3722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(71..76,77..92,93..99)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             224..1873
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(287..305)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      1930..2016
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2181..2769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2261..2280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2858..2952)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2973..3632)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3547..3566
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(3633..3722,1..13))
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tacgtgccga tcaacgtctc attttcgcca gatatcctcg agcgcggaat tccctagggg
       61 atccgagctg ttgacaatta atcatcggct cgtataatgt gtgggacgtc gcgacggtat
      121 agcgaccccg tctctgtgtg cgccagagac actggggcag atatgggcca gcacagaatg
      181 aggcccccta gtaactttac ggagggccag catggaagct ttcgaagacg ccaaaaacat
      241 aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag agcaactgca
      301 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat
      361 cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg cagaagctat
      421 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca
      481 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga
      541 catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt
      601 tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac caataatcca
      661 gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt
      721 cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag agtcctttga
      781 tcgtgacaaa acaattgcac tgataatgaa ttcctctgga tctactgggt tacctaaggg
      841 tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag atcctatttt
      901 tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt
      961 tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag
     1021 atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa gtgcgttgct
     1081 agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat acgatttatc
     1141 taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg gggaagcggt
     1201 tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg agactacatc
     1261 agctattctg attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc
     1321 attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcagag
     1381 aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca atccggaagc
     1441 gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga
     1501 cgaagacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat acaaaggata
     1561 tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca tcttcgacgc
     1621 gggcgtggca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt
     1681 ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtggcca gtcaagtaac
     1741 aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac
     1801 cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa
     1861 gtccaaattg taaccgcaca ccttactggt gtgcggacta gtccaactgc agaaaagcgg
     1921 ccgcggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1981 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagag catgcgcgtt
     2041 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2101 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2161 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2221 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2281 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2341 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2401 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2461 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2521 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2581 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     2641 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2701 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2761 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2821 tcacgttaag ggattttggt catgcccggg tgcttggatt ctcaccaata aaaaacgccc
     2881 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     2941 tcaacaggag tccaagcggt accgatatca aattacgccc cgccctgcca ctcatcgcag
     3001 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     3061 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     3121 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     3181 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
     3241 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     3301 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     3361 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
     3421 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     3481 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     3541 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     3601 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     3661 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     3721 ct
//
