LOCUS       sequence-106578-        3704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This pFTV1 vector contains a thyroxine binding riboswitch, 
            controlling the translation initaition rate of luciferase gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-T4-2
FEATURES             Location/Qualifiers
     source          1..3704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(71..76,77..92,93..99)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             206..1855
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(269..287)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      1912..1998
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2163..2751)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2243..2262)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2840..2934)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2955..3614)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3529..3548
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(3615..3704,1..13))
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tacgtgccga tcaacgtctc attttcgcca gatatcctcg agcgcggaat tccctagggg
       61 atccgagctg ttgacaatta atcatcggct cgtataatgt gtgggacgtc tcaagaaatc
      121 ggcgcgggag tcacggtttg tgggaggctg tggaggcgag accgtgacgt acctccgctt
      181 caggagggag acaatggaag ctttcgaaga cgccaaaaac ataaagaaag gcccggcgcc
      241 attctatcct ctagaggatg gaaccgctgg agagcaactg cataaggcta tgaagagata
      301 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtga acatcacgta
      361 cgcggaatac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
      421 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
      481 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
      541 attgctcaac agtatgaaca tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt
      601 gcaaaaaatt ttgaacgtgc aaaaaaaatt accaataatc cagaaaatta ttatcatgga
      661 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
      721 tcccggtttt aatgaatacg attttgtacc agagtccttt gatcgtgaca aaacaattgc
      781 actgataatg aattcctctg gatctactgg gttacctaag ggtgtggccc ttccgcatag
      841 aactgcctgc gtcagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
      901 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
      961 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
     1021 tttacgatcc cttcaggatt acaaaattca aagtgcgttg ctagtaccaa ccctattttc
     1081 attcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
     1141 ttctgggggc gcacctcttt cgaaagaagt cggggaagcg gttgcaaaac gcttccatct
     1201 tccagggata cgacaaggat atgggctcac tgagactaca tcagctattc tgattacacc
     1261 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
     1321 tgtggatctg gataccggga aaacgctggg cgttaatcag agaggcgaat tatgtgtcag
     1381 aggacctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
     1441 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
     1501 catagttgac cgcttgaagt ctttaattaa atacaaagga tatcaggtgg cccccgctga
     1561 attggaatcg atattgttac aacaccccaa catcttcgac gcgggcgtgg caggtcttcc
     1621 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
     1681 gacggaaaaa gagatcgtgg attacgtggc cagtcaagta acaaccgcga aaaagttgcg
     1741 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
     1801 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagtccaaat tgtaaccgca
     1861 caccttactg gtgtgcggac tagtccaact gcagaaaagc ggccgcggca tcaaataaaa
     1921 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1981 ctcctgagta ggacaaatcc gccgccctag agcatgcgcg ttcggctgcg gcgagcggta
     2041 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2101 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2161 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2221 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2281 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2341 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2401 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2461 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2521 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2581 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     2641 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2701 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2761 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2821 gtcatgcccg ggtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     2881 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     2941 gtaccgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     3001 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     3061 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     3121 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     3181 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     3241 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     3301 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     3361 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     3421 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     3481 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     3541 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     3601 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     3661 cccggtagtg atcttatttc attatggtga aagttggaac ctct
//
