LOCUS       sequence-106579-        1757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pUC19 vector contains a no-aptamer control mRNA encoding 
            luciferase gene. It should be used when testing AEB-TMR7 and 
            AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific 
            ligand effect on luciferase expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: Mishler2014-No-aptamer-controlluciferase-pUC19
FEATURES             Location/Qualifiers
     source          1..1757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(13..18,19..34,35..41)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             82..1734
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(148..166)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ggatccgagc tgttgacaat taatcatcgg ctcgtataat gtgtgggact cactataggt
       61 accctgagaa ggggcaacaa gatggaagac gccaaaaaca taaagaaagg cccggcgcca
      121 ttctatcctc tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac
      181 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac
      241 gcggaatact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat
      301 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg
      361 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa
      421 ttgctcaaca gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg
      481 caaaaaattt tgaacgtgca aaaaaaatta ccaataatcc agaaaattat tatcatggat
      541 tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct
      601 cccggtttta atgaatacga ttttgtacca gagtcctttg atcgtgacaa aacaattgca
      661 ctgataatga attcctctgg atctactggg ttacctaagg gtgtggccct tccgcataga
      721 actgcctgcg tcagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg
      781 gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc
      841 ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt
      901 ttacgatccc ttcaggatta caaaattcaa agtgcgttgc tagtaccaac cctattttca
      961 ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct
     1021 tctgggggcg cacctctttc gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt
     1081 ccagggatac gacaaggata tgggctcact gagactacat cagctattct gattacaccc
     1141 gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt
     1201 gtggatctgg ataccgggaa aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga
     1261 ggacctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac
     1321 aaggatggat ggctacattc tggagacata gcttactggg acgaagacga acacttcttc
     1381 atagttgacc gcttgaagtc tttaattaaa tacaaaggat atcaggtggc ccccgctgaa
     1441 ttggaatcga tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtcttccc
     1501 gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg
     1561 acggaaaaag agatcgtgga ttacgtggcc agtcaagtaa caaccgcgaa aaagttgcgc
     1621 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga
     1681 aaaatcagag agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaaccgcac
     1741 accttactgg tgtgcgg
//
