LOCUS       sequence-106580-        1802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pUC19 vector contains a tetramethylrosamine (TMR) binding 
            riboswitch, controlling the translation initaition rate of 
            luciferase gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-TMR7pUC19
FEATURES             Location/Qualifiers
     source          1..1802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(13..18,19..34,35..41)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     RBS             113..121
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             127..1779
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(193..211)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ggatccgagc tgttgacaat taatcatcgg ctcgtataat gtgtgggcag gctcccacgg
       61 atcgcgactg gcgagagcca ggtaacgaat cgatccagtt ccaccgattc gttaaggagg
      121 taatccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta tcctctagag
      181 gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga
      241 acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga atacttcgaa
      301 atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc
      361 gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc
      421 ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg
      481 aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac
      541 gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa aacggattac
      601 cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa
      661 tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat aatgaattcc
      721 tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc ctgcgtcaga
      781 ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta
      841 agtgttgttc cattccatca cggttttgga atgtttacta cactcggata tttgatatgt
      901 ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttttacg atcccttcag
      961 gattacaaaa ttcaaagtgc gttgctagta ccaaccctat tttcattctt cgccaaaagc
     1021 actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg gggcgcacct
     1081 ctttcgaaag aagtcgggga agcggttgca aaacgcttcc atcttccagg gatacgacaa
     1141 ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa
     1201 ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc
     1261 gggaaaacgc tgggcgttaa tcagagaggc gaattatgtg tcagaggacc tatgattatg
     1321 tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta
     1381 cattctggag acatagctta ctgggacgaa gacgaacact tcttcatagt tgaccgcttg
     1441 aagtctttaa ttaaatacaa aggatatcag gtggcccccg ctgaattgga atcgatattg
     1501 ttacaacacc ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga tgacgccggt
     1561 gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc
     1621 gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt
     1681 gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc
     1741 ctcataaagg ccaagaaggg cggaaagtcc aaattgtaac cgcacacctt actggtgtgc
     1801 gg
//
