LOCUS       sequence-106581-        1794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pUC19 vector contains a tetramethylrosamine (TMR) binding 
            riboswitch, controlling the translation initaition rate of 
            luciferase gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-TMR10pUC19
FEATURES             Location/Qualifiers
     source          1..1794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(13..18,19..34,35..41)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     RBS             106..114
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             119..1771
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(185..203)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ggatccgagc tgttgacaat taatcatcgg ctcgtataat gtgtgggatt cccgtaccgg
       61 atcgcgactg gcgagagcca ggtaacgaat cgatccaaga ttcgttaagg aggtccatat
      121 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatcctctag aggatggaac
      181 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
      241 ttttacagat gcacatatcg aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt
      301 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
      361 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
      421 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgaacatttc
      481 gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
      541 aaaattacca ataatccaga aaattattat catggattct aaaacggatt accagggatt
      601 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
      661 tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaatt cctctggatc
      721 tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca gattctcgca
      781 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
      841 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
      901 agtcgtctta atgtatagat ttgaagaaga gctgttttta cgatcccttc aggattacaa
      961 aattcaaagt gcgttgctag taccaaccct attttcattc ttcgccaaaa gcactctgat
     1021 tgacaaatac gatttatcta atttacacga aattgcttct gggggcgcac ctctttcgaa
     1081 agaagtcggg gaagcggttg caaaacgctt ccatcttcca gggatacgac aaggatatgg
     1141 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
     1201 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
     1261 gctgggcgtt aatcagagag gcgaattatg tgtcagagga cctatgatta tgtccggtta
     1321 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
     1381 agacatagct tactgggacg aagacgaaca cttcttcata gttgaccgct tgaagtcttt
     1441 aattaaatac aaaggatatc aggtggcccc cgctgaattg gaatcgatat tgttacaaca
     1501 ccccaacatc ttcgacgcgg gcgtggcagg tcttcccgac gatgacgccg gtgaacttcc
     1561 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
     1621 cgtggccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
     1681 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
     1741 ggccaagaag ggcggaaagt ccaaattgta accgcacacc ttactggtgt gcgg
//
