LOCUS       sequence-106593-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RPL16a HA-TEV-AVI pop-in/pop-out plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Jan CH, Williams CC, Weissman JS
  TITLE     Principles of ER cotranslational translocation revealed by 
            proximity-specific ribosome profiling.
  JOURNAL   Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521.
            Epub 2014 Nov 6.
  PUBMED    25378630
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-7"; volume: "346(6210):1257521. doi"; pages: " 
            10.1126/science.1257521. Epub 2014 Nov 6"
COMMENT     Sequence Label: pJW1505
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(345..389)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(393..413)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(423..449)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc tagaactagt
       61 gggcctatct gcaaacttag tacgggccat tccatccact tgcattacct tttgtgtata
      121 cgagaacctt aagcataggc tttagtaaat aaaggctata tgatagattt aatttcttgt
      181 aaatatcaaa cgataaaaat ataaaaagta cgaaagaaaa aggggaggta atctaaactg
      241 taacggggag gaatggtttc tttggttttt attatttcta aaaaatattt gtgattatat
      301 tacaaaatgt aaagtattaa agaaaaacta tttttaattt cttactcatg ccattcaatt
      361 ttttgagctt caaaaatatc attcaaacct gatccttgaa aatacaaatt ttctgaacct
      421 ccagcgtagt ctgggacgtc gtatgggtaa gaaccgtaac ccaaagcggc taattgttta
      481 gcaacatcag attcagcagc agtagcatta gcagaggcaa ctttcttggt gaaagctctc
      541 ttcttggcat agtattcagc ggatgaaacc tttctctttg cttccaattt ggcaacaaca
      601 tcttcgtatt tccaaccaac ggaagtagac aacttaccca aggtggtgta ctttctacct
      661 ggcttcaatc tcaaaactct caatgcttgt gggacaacaa ctctcttctt cttatcgtat
      721 ggaggtggga taccttcaaa gacctttaaa cgttccaaag cggccttacc acgagcagtc
      781 ttgtgagaaa ccataccacg aagagctttg tagaagattc tagatggggc tctgaa
//
