LOCUS       sequence-106600-       13552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Filamin with C-term stFRET inserted to measure 
            mechanical stress in real time.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13552)
  AUTHORS   Meng F, Suchyna TM, Sachs F
  TITLE     A fluorescence energy transfer-based mechanical stress sensor for 
            specific proteins in situ.
  JOURNAL   FEBS J. 2008 Jun;275(12):3072-87. doi: 
            10.1111/j.1742-4658.2008.06461.x. Epub 2008 May 10.
  PUBMED    18479457
REFERENCE   2  (bases 1 to 13552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2008.06461.x"; journalName: "FEBS J"; date: 
            "2008-06"; volume: "275"; issue: "12"; pages: "3072-87"
COMMENT     Sequence Label: Filamin-C-stFRET
FEATURES             Location/Qualifiers
     source          1..13552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(8568..8570,8571..9281)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(9405..9407,9408..9410,9411..10121)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             10122..10145
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     polyA_signal    10340..10461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10377..10396)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10431..10450
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(10468..10923)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10605..10626)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10817..10836
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10950..11054
                     /gene="bla"
                     /label=AmpR promoter
     promoter        11056..11413
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        11056..11247
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(11079..11099)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      11264..11399
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11448..12242
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             11448..12242
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11502..11521)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12112..12131
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(12430..12449)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12474..12521
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12850..13438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13339..13358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatga
      601 gtagctccca ctctcgggcg ggccagagcg cagcaggcgc ggctccgggc ggcggcgtcg
      661 acacgcggga cgccgagatg ccggccaccg agaaggacct ggcggaggac gcgccatgga
      721 agaagatcca gcagaacact ttcacgcgct ggtgcaacga gcacctgaag tgcgtgagca
      781 agcgcatcgc caacctgcag acggacctga gcgacgggct gcggcttatc gcgctgttgg
      841 aggtgctcag ccagaagaag atgcaccgca agcacaacca gcggcccact ttccgccaaa
      901 tgcagcttga gaacgtgtcg gtggcactcg agttcctgga ccgcgagagc atcaaactgg
      961 tgtccatcga cagcaaggcc atcgtggacg ggaacctgaa gctgatcctg ggcctcatct
     1021 ggaccctgat cctgcactac tccatctcca tgcccatgtg ggacgaggag gaggatgagg
     1081 aggccaagaa gcagaccccc aagcagaggc tcctgggctg gatccagaac aagctgccgc
     1141 agctgcccat caccaacttc agccgggact ggcagagcgg ccgggccctg ggcgccctgg
     1201 tggacagctg tgccccgggc ctgtgtcctg actgggactc ttgggacgcc agcaagcccg
     1261 ttaccaatgc gcgagaggcc atgcagcagg cggatgactg gctgggcatc ccccaggtga
     1321 tcacccccga ggagattgtg gaccccaacg tggacgagca ctctgtcatg acctacctgt
     1381 cccagttccc caaggccaag ctgaagccag gggctccctt gcggcccaaa ctgaacccga
     1441 agaaagcccg tgcctacggg ccaggcatcg agcccacagg caacatggtg aagaagcggg
     1501 cagagttcac tgtggagacc agaagtgctg gccagggaga ggtgctggtg tacgtggagg
     1561 acccggccgg acaccaggag gaggcaaaag tgaccgccaa taacgacaag aaccgcacct
     1621 tctccgtctg gtacgtcccc gaggtgacgg ggactcataa ggttactgtg ctctttgctg
     1681 gccagcacat cgccaagagc cccttcgagg tgtacgtgga taagtcacag ggtgacgcca
     1741 gcaaagtgac agcccaaggt cccggcctgg agcccagtgg caacatcgcc aacaagacca
     1801 cctactttga gatctttacg gcaggagctg gcacgggcga ggtcgaggtt gtgatccagg
     1861 accccatggg acagaagggc acggtagagc ctcagctgga ggcccggggc gacagcacat
     1921 accgctgcag ctaccagccc accatggagg gcgtccacac cgtgcacgtc acgtttgccg
     1981 gcgtgcccat ccctcgcagc ccctacactg tcactgttgg ccaagcctgt aacccgagtg
     2041 cctgccgggc ggttggccgg ggcctccagc ccaagggtgt gcgggtgaag gagacagctg
     2101 acttcaaggt gtacacaaag ggcgctggca gtggggagct gaaggtcacc gtgaagggcc
     2161 ccaagggaga ggagcgcgtg aagcagaagg acctggggga tggcgtgtat ggcttcgagt
     2221 attaccccat ggtccctgga acctatatcg tcaccatcac gtggggtggt cagaacatcg
     2281 ggcgcagtcc cttcgaagtg aaggtgggca ccgagtgtgg caatcagaag gtacgggcct
     2341 ggggccctgg gctggagggc ggcgtcgttg gcaagtcagc agactttgtg gtggaggcta
     2401 tcggggacga cgtgggcacg ctgggcttct cggtggaagg gccatcgcag gctaagatcg
     2461 aatgtgacga caagggcgac ggctcctgtg atgtgcgcta ctggccgcag gaggctggcg
     2521 agtatgccgt tcacgtgctg tgcaacagcg aagacatccg cctcagcccc ttcatggctg
     2581 acatccgtga cgcgccccag gacttccacc cagacagggt gaaggcacgt gggcctggat
     2641 tggagaagac aggtgtggcc gtcaacaagc cagcagagtt cacagtggat gccaagcacg
     2701 gtggcaaggc cccacttcgg gtccaagtcc aggacaatga aggctgccct gtggaggcgt
     2761 tggtcaagga caacggcaat ggcacttaca gctgctccta cgtgcccagg aagccggtga
     2821 agcacacagc catggtgtcc tggggaggcg tcagcatccc caacagcccc ttcagggtga
     2881 atgtgggagc tggcagccac cccaacaagg tcaaagtata cggccccgga gtagccaaga
     2941 cagggctcaa ggcccacgag cccacctact tcactgtgga ctgcgccgag gctggccagg
     3001 gggacgtcag catcggcatc aagtgtgccc ctggagtggt aggccccgcc gaagctgaca
     3061 tcgacttcga catcatccgc aatgacaatg acaccttcac ggtcaagtac acgccccggg
     3121 gggctggcag ctacaccatt atggtcctct ttgctgacca ggccacgccc accagcccca
     3181 tccgagtcaa ggtggagccc tctcatgacg ccagtaaggt gaaggccgag ggccctggcc
     3241 tcagtcgcac tggtgtcgag cttggcaagc ccacccactt cacagtaaat gccaaagctg
     3301 ctggcaaagg caagctggac gtccagttct caggactcac caagggggat gcagtgcgag
     3361 atgtggacat catcgaccac catgacaaca cctacacagt caagtacacg cctgtccagc
     3421 agggtccagt aggcgtcaat gtcacttatg gaggggatcc catccctaag agccctttct
     3481 cagtggcagt atctccaagc ctggacctca gcaagatcaa ggtgtctggc ctgggagaga
     3541 aggtggacgt tggcaaagac caggagttca cagtcaaatc aaagggtgct ggtggtcaag
     3601 gcaaagtggc atccaagatt gtgggcccct cgggtgcagc ggtgccctgc aaggtggagc
     3661 caggcctggg ggctgacaac agtgtggtgc gcttcctgcc ccgtgaggaa gggccctatg
     3721 aggtggaggt gacctatgac ggcgtgcccg tgcctggcag cccctttcct ctggaagctg
     3781 tggcccccac caagcctagc aaggtgaagg cgtttgggcc ggggctgcag ggaggcagtg
     3841 cgggctcccc cgcccgcttc accatcgaca ccaagggcgc cggcacaggt ggcctgggcc
     3901 tgacggtgga gggcccctgt gaggcgcagc tcgagtgctt ggacaatggg gatggcacat
     3961 gttccgtgtc ctacgtgccc accgagcccg gggactacaa catcaacatc ctcttcgctg
     4021 acacccacat ccctggctcc ccattcaagg cccacgtggt tccctgcttt gacgcatcca
     4081 aagtcaagtg ctcaggcccc gggctggagc gggccaccgc tggggaggtg ggccaattcc
     4141 aagtggactg ctcgagcgcg ggcagcgcgg agctgaccat tgagatctgc tcggaggcgg
     4201 ggcttccggc cgaggtgtac atccaggacc acggtgatgg cacgcacacc attacctaca
     4261 ttcccctctg ccccggggcc tacaccgtca ccatcaagta cggcggccag cccgtgccca
     4321 acttccccag caagctgcag gtggaacctg cggtggacac ttccggtgtc cagtgctatg
     4381 ggcctggtat tgagggccag ggtgtcttcc gtgaggccac cactgagttc agtgtggacg
     4441 cccgggctct gacacagacc ggagggccgc acgtcaaggc ccgtgtggcc aacccctcag
     4501 gcaacctgac ggagacctac gttcaggacc gtggcgatgg catgtacaaa gtggagtaca
     4561 cgccttacga ggagggactg cactccgtgg acgtgaccta tgacggcagt cccgtgccca
     4621 gcagcccctt ccaggtgccc gtgaccgagg gctgcgaccc ctcccgggtg cgtgtccacg
     4681 ggccaggcat ccaaagtggc accaccaaca agcccaacaa gttcactgtg gagaccaggg
     4741 gagctggcac gggcggcctg ggcctggctg tagagggccc ctccgaggcc aagatgtcct
     4801 gcatggataa caaggacggc agctgctcgg tcgagtacat cccttatgag gctggcacct
     4861 acagcctcaa cgtcacctat ggtggccatc aagtgccagg cagtcctttc aaggtccctg
     4921 tgcatgatgt gacagatgcg tccaaggtca agtgctctgg gcccggcctg agcccaggca
     4981 tggttcgtgc caacctccct cagtccttcc aggtggacac aagcaaggct ggtgtggccc
     5041 cattgcaggt caaagtgcaa gggcccaaag gcctggtgga gccagtggac gtggtagaca
     5101 acgctgatgg cacccagacc gtcaattatg tgcccagccg agaagggccc tacagcatct
     5161 cagtactgta tggagatgaa gaggtacccc ggagcccctt caaggtcaag gtgctgccta
     5221 ctcatgatgc cagcaaggtg aaggccagtg gccccgggct caacaccact ggcgtgcctg
     5281 ccagcctgcc cgtggagttc accatcgatg caaaggacgc cggggagggc ctgctggctg
     5341 tccagatcac ggatcccgaa ggcaagccga agaagacaca catccaagac aaccatgacg
     5401 gcacgtatac agtggcctac gtgccagacg tgacaggtcg ctacaccatc ctcatcaagt
     5461 acggtggtga cgagatcccc ttctccccgt accgcgtgcg tgccgtgccc accggggacg
     5521 ccagcaagtg cactgtcaca gtgtcaatcg gaggtcacgg gctaggtgct ggcatcggcc
     5581 ccaccattca gattggggag gagacggtga tcactgtgga cactaaggcg gcaggcaaag
     5641 gcaaagtgac gtgcaccgtg tgcacgcctg atggctcaga ggtggatgtg gacgtggtgg
     5701 agaatgagga cggcactttc gacatcttct acacggcccc ccagccgggc aaatacgtca
     5761 tctgtgtgcg ctttggtggc gagcacgtgc ccaacagccc cttccaagtg acggctctgg
     5821 ctggggacca gccctcggtg cagccccctc tacggtctca gcagctggcc ccacagtaca
     5881 cctacgccca gggcggccag cagacttggg ccccggagag gcccctggtg ggtgtcaatg
     5941 ggctggatgt gaccagcctg aggccctttg accttgtcat ccccttcacc atcaagaagg
     6001 gcgagatcac aggggaggtt cggatgccct caggcaaggt ggcgcagccc accatcactg
     6061 acaacaaaga cggcaccgtg accgtgcggt atgcacccag cgaggctggc ctgcacgaga
     6121 tggacatccg ctatgacaac atgcacatcc caggaagccc cttgcagttc tatgtggatt
     6181 acgtcaactg tggccatgtc actgcctatg ggcctggcct cacccatgga gtagtgaaca
     6241 agcctgccac cttcaccgtc aacaccaagg atgcaggaga ggggggcctg tctctggcca
     6301 ttgagggccc gtccaaagca gaaatcagct gcactgacaa ccaggatggg acatgcagcg
     6361 tgtcctacct gcctgtgctg ccgggggact acagcattct agtcaagtac aatgaacagc
     6421 acgtcccagg cagccccttc actgctcggg tcacaggtga cgactccatg cgtatgtccc
     6481 acctaaaggt cggctctgct gccgacatcc ccatcaacat ctcagagacg gatctcagcc
     6541 tgctgacggc cactgtggtc ccgccctcgg gccgggagga gccctgtttg ctgaagcggc
     6601 tgcgtaatgg ccacgtgggg atttcattcg tgcccaagga gacgggggag cacctggtgc
     6661 atgtgaagaa aaatggccag cacgtggcca gcagccccat cccggtggtg atcagccagt
     6721 cggaaattgg ggatgccagt cgtgttcggg tctctggtca gggccttcac gaaggccaca
     6781 cctttgagcc tgcagagttt atcattgata cccgcgatgc aggctatggt gggctcagcc
     6841 tgtccattga gggccccagc aaggtggaca tcaacacaga ggacctggag gacgggacgt
     6901 gcagggtcac ctactgcccc acagagccag gcaactacat catcaacatc aagtttgccg
     6961 accagcacgt gcctggcagc cccttctctg tgaaggtgac aggcgagggc cgggtgaaag
     7021 agagcatcac ccgcaggcgt cgggctcctt cagtggccaa cgttggtagt cattgtgacc
     7081 tcagcctgaa aatccctgaa attagcatcc aggatatgac agcccaggtg accagcccat
     7141 cgggcaagac ccatgaggcc gagatcgtgg aaggggagaa ccacacctac tgcatccgct
     7201 ttgttcccgc tgagatgggc acacacacag tcagcgtcaa gtacaagggc cagcacgtgc
     7261 ctgggagccc cttccagttc accgtggggc ccctagggga agggggagcc cacaaggtcc
     7321 gagctggggg ccctggcctg gagagagctg aagctggagt gccagccgaa ttcagtatct
     7381 ggacccggga agctggtgct ggaggcctgg ccattgctgt cgagggcccc agcaaggctg
     7441 agatctcttt tgaggaccgc aaggacggct cctgtggtgt ggcttatgtg gtccaggagc
     7501 caggtgacta cgaagtctca gtcaagttca acgaggaaca cattcccgac agccccttcg
     7561 tggtgcctgt ggcttctccg tctggcgacg cccgccgcct cactgtttct agccttcagg
     7621 agtcagggct aaaggtcaac cagccagcct cttttgcagt cagcctgaac ggggccaagg
     7681 gggcgatcga tgccaaggtg cacagcccct caggagccct ggaggagtgc tatgtcacag
     7741 aaattgacca agataagtat gctgtgcgct tcatccctcg ggagaatggc gtttacctga
     7801 ttgacgtcaa gttcaacggc acccacatcc ctggaagccc cttcaagatc cgagttgggg
     7861 agcctgggca tggaggggac ccaggcttgg tgtctgctta cggagcaggt ctggaaggcg
     7921 gtgtcacagg gaacccagct gagttcgtcg tgaacacgag caatgcggga gctggtgccc
     7981 tgtcggtgac cattgacggc ccctccaagg tgaagatgga ttgccaggag tgccctgagg
     8041 gctaccgcgt cacctatacc cccatggcac ctggcagcta cctcatctcc atcaagtacg
     8101 gcggccccta ccacattggg ggcagcccct tcaaggccaa agtcacaggc ccccgtctcg
     8161 tcagcaacca cagcctccac gagacatcat cagtgtttgt agactctctg accaaggcca
     8221 cctgtgcccc ccagcatggg gccccgggtc ctgggcctgc tgacgccagc aaggtggtgg
     8281 ccaagggcct ggggctgagc aaggcctacg taggccagaa gagcagcttc acagtagact
     8341 gcagcaaagc aggcaacaac atgctgctgg tgggggttca tggcccaagg accccctgcg
     8401 aggagatcct ggtgaagcac gtgggcagcc ggctctacag cgtgtcctac ctgctcaagg
     8461 acaaggggga gtacacactg gtggtcaaat gggggcacga gcacatccca ggcagccccc
     8521 accgcgttgt ggtgcccacc ggtggcggaa agcttcagct gggatccgtg agcaagggcg
     8581 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     8641 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     8701 agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg
     8761 gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     8821 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     8881 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     8941 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     9001 acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
     9061 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga
     9121 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     9181 ccaaactgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     9241 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc agatctgggc
     9301 tgcgcaagcg cttacgaaaa tttagaaaca agattaaaga aaagcttaaa aaaattggtc
     9361 agaaaatcca gggtttcgtg ccgatacttg caggtgtgaa ttccatggtg agcaagggcg
     9421 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     9481 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     9541 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     9601 cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     9661 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     9721 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     9781 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacg
     9841 ccatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
     9901 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     9961 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
    10021 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
    10081 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gcatcatcac caccatcatc
    10141 accacggagg tggcagcggc cgctaagagc tcaagcttcg aattctgcag tcgacggtac
    10201 cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
    10261 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    10321 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    10381 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10441 caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg
    10501 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
    10561 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
    10621 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
    10681 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
    10741 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
    10801 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
    10861 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
    10921 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    10981 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    11041 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
    11101 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    11161 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    11221 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
    11281 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    11341 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    11401 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
    11461 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
    11521 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
    11581 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
    11641 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
    11701 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
    11761 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
    11821 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
    11881 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
    11941 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
    12001 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
    12061 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
    12121 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
    12181 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
    12241 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
    12301 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
    12361 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
    12421 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
    12481 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
    12541 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
    12601 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
    12661 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
    12721 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    12781 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    12841 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    12901 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    12961 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
    13021 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    13081 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    13141 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    13201 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    13261 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    13321 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    13381 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    13441 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    13501 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
